Identification of intestinal bacterial flora in Rhipicephalus microplus ticks by conventional methods and PCR-DGGE analysis
Identification of intestinal bacterial flora in Rhipicephalus microplus ticks by conventional methods and PCR-DGGE analysis
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常规方法鉴定微小扇头蜱肠道菌群及PCR-DGGE分析
DOI：
10.1007/s10493-015-9896-1
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发表时间：
2015
影响因子：
2.2
通讯作者：
Yan Fen
中科院分区：
文献类型：
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作者：
Xu Xing-Li;Cheng Tian-Yin;Yang Hu;Yan Fen
本研究采用基于PCR和变性梯度凝胶电泳（PCR-DGGE）的培养依赖和非培养依赖两种方法，对微扇头蜱肠道菌群植物群进行了分析。TheR.从中国江西、湖南和贵州省的牛和山羊中收集微plusticks。使用培养依赖性方法分离出三种不同的细菌菌株：模拟葡萄球菌、枯草芽孢杆菌和弯曲芽孢杆菌菌株。PCR-DGGE分析发现19条不同的DGGE带，对它们的鉴定表明它们属于立克次体科、黄单胞菌科、柯克斯体属、Ehrlichiasp.，假单胞菌属，Ehrlichiasp.，Orphnebiussp.，孔雀立克次体、弯曲芽孢杆菌、孔雀立克次体和柯克斯体为优势菌。牛微孢子虫、假单胞菌属（Pseudomonassp.）和B.山羊蜱类中以弯曲株最常见，犬埃立克体仅在R.本文报道了湖南永顺地区的微plusticks。结果表明，红腹锦鸡肠道微生物多样性较低，而红腹锦鸡肠道微生物多样性较低。蜱类宿主和采样地点的地方性差异影响微plusticks，这两个方面可能会影响病原体向人和动物的传播。
In this study, we have analyzed the intestinal microbial flora associated withRhipicephalus microplusticks using both culture-dependent and independent methods based on PCR and denaturing gradient gel electrophoresis (PCR–DGGE). TheR. microplusticks were collected from cattle and goats in Jiangxi, Hunan and Guizhou Provinces of China. Three distinct strains of bacteria were isolated using culture-dependent methods:Staphylococcus simulans,Bacillus subtilisandBacillus flexusstrain. Nineteen distinct DGGE bands were found using PCR–DGGE analysis, and their search for identity shows that they belonged to Rickettsiaceae, Xanthomonadaceae,Coxiellasp.,Ehrlichiasp.,Pseudomonassp.,Ehrlichiasp.,Orphnebiussp.,Rickettsia peacockii,Bacillus flexus.Rickettsia peacockiiandCoxiellagenus were the dominant strain of theR. microplusticks from cattle,Pseudomonassp. andB. flexusstrain were the most common species in all tick samples from goats.Ehrlichia caniswere detected only inR. microplusticks from Yongshun area in Hunan Province. The results indicate that the intestinal microbial diversity ofR. microplusticks was influenced by tick hosts and local differences in the sampling location and these two aspects may affect transmission of pathogen to humans and animals.