Metalloestrogenic effects of quantum dots
Metalloestrogenic effects of quantum dots
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DOI：
10.2217/nnm.11.102
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发表时间：
2012-01-01
期刊：
影响因子：
5.5
通讯作者：
Maysinger, Dusica
中科院分区：
文献类型：
--
作者：
Jain, Manasi P.;Vaisheva, Farida;Maysinger, Dusica
目的：研究碲化镉量子点（QDs）在人乳腺癌细胞和小鼠体内的金属雌激素样作用。材料和方法：利用人乳腺癌细胞（MCF-7细胞）研究量子点、镉和17 β-雌二醇诱导的雌激素相关的基因组和非基因组信号传导。雌性青春期前和卵巢切除的成年小鼠用CdTe量子点处理，以评估量子点诱导的雌激素活性是否会导致子宫变化。结果与讨论：我们的研究结果表明，在体外含镉量子点诱导细胞增殖，雌激素受体α激活，和双相磷酸化的AKT和ERK 1/2，与17 β-雌二醇。绿色量子点比橙子量子点引起更强的雌激素反应。添加选择性雌激素受体拮抗剂ICI 182780，完全消除了所有QD诱导的雌激素效应，表明QD诱导的雌激素信号是通过雌激素受体介导的。在体内，用QD长期治疗小鼠导致子宫重量增加2至3倍，相当于或大于17 β-雌二醇。结论：这些发现表明，某些含镉纳米晶体是内分泌干扰物，其影响可能超过离子镉或17 β-雌二醇诱导的影响。
Aim: To investigate the metalloestrogenic effects of cadmium telluride quantum dots (QDs) in both human breast cancer cells and in vivo in mice. Materials & Methods: Human breast cancer cells (MCF-7 cells) were utilized to study QDs, cadmium and 17 beta-estradiol induced estrogen-related genomic and nongenomic signaling. Female prepubescent and ovariectomized adult mice were treated with CdTe QDs to assess whether QD-induced estrogenicity would lead to uterine changes. Results & Discussion: Our findings demonstrate that in vitro cadmium-containing QDs induce cellular proliferation, estrogen receptor alpha activation, and biphasic phosphorylation of AKT and ERK1/2, comparable with 17 beta-estradiol. Green QDs elicited a more robust estrogenic response than orange QDs. Addition of the selective estrogen receptor antagonist, ICI 182780, completely abolished all QD-induced estrogenic effects, suggesting that QD-induced estrogenic signaling is mediated via the estrogen receptor. In vivo, chronic treatment of mice with QDs led to a two- to three-fold increase in uterine weight, comparable or greater than 17 beta-estradiol. Conclusion: These findings suggest that certain cadmium-containing nanocrystals are endocrine disruptors, whose effects can exceed those induced by ionic cadmium or 17 beta-estradiol.