In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes
In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes
复制标题
DOI：
10.1016/j.biomaterials.2010.10.030
复制
发表时间：
2011-02-01
期刊：
影响因子：
14
通讯作者：
Trajkovic, Vladimir S.
中科院分区：
文献类型：
--
作者：
Markovic, Zoran M.;Harhaji-Trajkovic, Ljubica M.;Trajkovic, Vladimir S.
本研究比较了近红外（NIR）激发的石墨烯纳米颗粒和碳纳米管（CNT）的光热抗癌活性。尽管近红外吸收能力较低，但在相同条件下，聚乙烯吡咯烷酮涂覆的石墨烯片悬浮液暴露在近红外辐射（808 nm, 2 W/cm(2)）下产生的热量比DNA或十二烷基苯磺酸钠溶解的单壁碳纳米管更多。因此，石墨烯纳米颗粒在体外诱导U251人胶质瘤细胞光热死亡方面的表现明显优于碳纳米管。考虑到两种碳纳米颗粒的热力学、光学和几何性质，一个简单的计算可以支持石墨烯片优越的光热灵敏度，这在很大程度上可以通过其更好的分散性来解释。石墨烯介导的光热杀伤癌细胞的机制显然涉及氧化应激和线粒体膜去极化，分别导致以caspase激活/DNA断裂和细胞膜损伤为特征的混合凋亡和坏死细胞死亡。(c) 2010 Elsevier Ltd.版权所有。
The present study compared the photothermal anticancer activity of near-infrared (NIR)-excited graphene nanoparticles and carbon nanotubes (CNT). Despite lower NIR-absorbing capacity, suspension of polyvinylpyrrolidone-coated graphene sheets exposed to NIR radiation (808 nm, 2 W/cm(2)) generated more heat than DNA or sodium dodecylbenzenesulfonate-solubilized single-wall CNT under the same conditions. Accordingly, graphene nanoparticles performed significantly better than CNT in inducing photothermal death of U251 human glioma cells in vitro. The superior photothermal sensitivity of graphene sheets could be largely explained by their better dispersivity, which has been supported by a simple calculation taking into account thermodynamic, optical and geometrical properties of the two type of carbon nanoparticles. The mechanisms of graphene-mediated photothermal killing of cancer cells apparently involved oxidative stress and mitochondrial membrane depolarization resulting in mixed apoptotic and necrotic cell death characterized by caspase activation/DNA fragmentation and cell membrane damage, respectively. (c) 2010 Elsevier Ltd. All rights reserved.