Ancient evolutionary origin of the neural crest gene regulatory network
Ancient evolutionary origin of the neural crest gene regulatory network
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DOI：
10.1016/j.devcel.2007.08.005
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发表时间：
2007-09-01
影响因子：
11.8
通讯作者：
Bronner-Fraser, Marianne
中科院分区：
文献类型：
--
作者：
Sauka-Spengler, Tatjana;Meulemans, Daniel;Bronner-Fraser, Marianne
脊椎动物的神经脊从它的起源神经板边缘迁移，形成不同的衍生品。我们先前假设神经脊基因调控网络(NC-GRN)引导神经脊的形成。在这里，我们通过分析现存基本脊椎动物七鳃鳗的神经脊形成来研究在进化过程中这个假设的网络是什么时候出现的。我们鉴定了50个NC-GRN同源物，并使用吗啉来证明8个转录调控因子的关键作用。这些结果揭示了上游因子的配置上的保守性，表明该网络的近端部分在脊椎动物进化的早期就出现了，并且已经保守了5亿年。我们发现神经脊说明符的双向表达，以及一些有望赋予物种特异性属性的说明符和效应符的部署上的差异。通过测试单个基础脊椎动物中神经脊基因的集体表达和功能，我们揭示了NC-GRN的基态，并解决了模式生物之间的歧义。
The vertebrate neural crest migrates from its origin, the neural plate border, to form diverse derivatives. We previously hypothesized that a neural crest gene regulatory network (NC-GRN) guides neural crest formation. Here, we investigate when during evolution this hypothetical network emerged by analyzing neural crest formation in lamprey, a basal extant vertebrate. We identify 50 NC-GRN homologs and use morpholinos to demonstrate a critical role for eight transcriptional regulators. The results reveal conservation in deployment of upstream factors, suggesting that proximal portions of the network arose early in vertebrate evolution and have been conserved for >500 million years. We found biphasic expression of neural crest specifiers and differences in deployment of some specifiers and effectors expected to confer species-specific properties. By testing the collective expression and function of neural crest genes in a single, basal vertebrate, we reveal the ground state of the NC-GRN and resolve ambiguities between model organisms.