Ultrastructural criteria for identification of mast cells and basophils in humans, guinea pigs, and mice.
Ultrastructural criteria for identification of mast cells and basophils in humans, guinea pigs, and mice.
复制标题
鉴定人类、豚鼠和小鼠肥大细胞和嗜碱性粒细胞的超微结构标准。
DOI：
10.1164/arrd.1983.128.2p2.s49
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Galli,SJ
中科院分区：
文献类型：
--
作者：
Dvorak,AM;Dvorak,HF;Galli,SJ
嗜碱性粒细胞和肥大细胞代表不同的群体，它们可能在功能、生化和对药物的反应方面表现出差异。在这篇综述中，我们定义了有助于区分人、豚鼠和小鼠的嗜碱性粒细胞和肥大细胞的超微结构标准。虽然正常成熟嗜碱性粒细胞和肥大细胞的超微结构特征是本报告的主要主题，但我们也描述了反映细胞功能活动或成熟阶段的嗜碱性粒细胞和肥大细胞超微结构的变化。这种超微结构的变化可以显著改变这些细胞的外观，对识别炎症部位的嗜碱性粒细胞和肥大细胞尤为重要。除了促进体内嗜碱性粒细胞和肥大细胞的准确识别外，超微结构分析在体外研究嗜碱性粒细胞和肥大细胞的成熟和功能方面发挥了重要作用。这些细胞的形态和生物化学现在可以通过分析来自血液或组织(特别是肺)的高度纯化的群体，或者通过检查体外维持的克隆细胞系来研究。利用这些重要的技术进步有望迅速增加我们对嗜碱性粒细胞和肥大细胞在健康和疾病中的作用的了解。
Basophils and mast cells represent distinct populations that may express differences in function, biochemistry, and response to drugs. In this review, we define ultrastructural criteria useful in distinguishing basophils from mast cells in humans, the guinea pig, and the mouse. Although the ultrastructural features of normal mature basophils and mast cells are the main subject of this report, we also describe variations in basophil and mast cell ultrastructure that reflect the cells' functional activity or stage of maturation. Appreciation of such ultrastructural changes, which can strikingly alter the appearance of these cells, is particularly important in identifying basophils and mast cells in sites of inflammation. In addition to facilitating the accurate identification of basophils and mast cellsin vivo, ultrastructural analysis has played an important role inin vitrostudies of basophil and mast cell maturation and function. The morphology and biochemistry of these cells now can be studied either by analyzing highly purified populations derived from blood or tissues, notably the lung, or by examining cloned cell lines maintainedin vitro. Exploitation of these important technical advances promises to rapidly increase our understanding of the roles of basophils and mast cells in health and disease.