Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.
Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.
复制标题
DOI：
10.1021/bc900302t
复制
发表时间：
2009-10-21
影响因子：
4.7
通讯作者：
Belfield KD
中科院分区：
文献类型：
--
作者：
Morales AR;Yanez CO;Schafer-Hales KJ;Marcus AI;Belfield KD
与非线性生物成像的发展密切相关的是用于研究生物功能和活性的光学探针的发展。引入具有增强的非线性的新的荧光化合物对于推进生物科学中的双光子吸收（2 PA）过程的实用性是必不可少的。在这里，我们报告的芴为基础的荧光团的合成量身定制的多光子成像，将琥珀酰亚胺酯和硫酯功能作为反应性接头，进一步耦合各种各样的生物相关分子。琥珀酰亚胺酯胺反应性探针与环肽RGDfK和多克隆抗大鼠IgG IgG蛋白缀合。在缀合后，活性2 PA发色团的基本分子结构和生物物理性质保持不变。证明了与芴-RGD肽缀合物或芴-IgG缀合物孵育的COS-7和HeLa细胞的常规和双光子荧光显微镜（2 PFM）成像。芴-IgG缀合物用于在早期有丝分裂发育阶段对细胞纺锤体成像。
Closely involved in the progression of nonlinear bioimaging is the development of optical probes for investigating biological function and activity. Introduction of new fluorescent compounds possessing enhanced nonlinearities are essential for advancing the utility of two-photon absorption (2PA) processes in the biological sciences. Herein, we report the synthesis of fluorene-based fluorophores tailored for multiphoton imaging, incorporating the succinimidyl ester and thioester functionality as reactive linkers for further coupling with a wide variety of biologically relevant molecules. The succinimidyl ester amine reactive probe was conjugated with the cyclic peptide RGDfK and polyclonal anti-rat IgG IgG protein. Upon conjugation, the basic molecular architecture and photophysical properties of the active 2PA chromophore remain unchanged. Conventional and two-photon fluorescence microscopy (2PFM) imaging of COS-7 and HeLa cells, incubated with either the fluorene-RGD peptide conjugate or the fluorene-IgG conjugate, was demonstrated. The fluorene-IgG conjugate was used to image cell spindles at early mitotic developmental stages.
登录
查看更多内容
DOI：
10.1385/0-89603-308-2:205
发表时间：
1995-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Haugland, R P
通讯作者：
Haugland, R P
影响因子：
2.7
作者：
Meltola, NJ;Wahlroos, R;Soini, AE
通讯作者：
Soini, AE
影响因子：
2.7
作者：
Meltola, NJ;Soini, AE;Hänninen, PE
通讯作者：
Hänninen, PE
影响因子：
16.2
作者：
Dulabon, L;Olson, EC;Anton, ES
通讯作者：
Anton, ES
影响因子：
2.8
作者：
Fakis, M;Polyzos, J;Mikroyannidis, J
通讯作者：
Mikroyannidis, J