Genome-Wide Mapping of Loci Associated With Resistance to Clubroot inBrassica napusssp.napobrassica(Rutabaga) Accessions From Nordic Countries
Genome-Wide Mapping of Loci Associated With Resistance to Clubroot inBrassica napusssp.napobrassica(Rutabaga) Accessions From Nordic Countries
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DOI：
10.3389/fpls.2020.00742
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发表时间：
2020-06-12
影响因子：
5.6
通讯作者：
Strelkov, Stephen E.
中科院分区：
文献类型：
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作者：
Fredua-Agyeman, Rudolph;Yu, Zhiyu;Strelkov, Stephen E.
芜菁甘蓝[芸苔]据报道，Hanelt是一种棒根（Plasmodiophora brassicae）抗性基因的优良来源。在这项研究中，对来自北欧国家（挪威、瑞典、芬兰、丹麦和冰岛）的124株芜菁甘蓝进行了评价，分析了它们对代表p。油菜病型2F、3H、5I、6M、8N和12个田间分离株，分别代表病型2B、3A、30、5C、5G、5K、5L、5X（2个分离株，L-G2和L-G3）、8E、8J和8P。利用15K的芸苔SNP阵列和60个pcr引物，对这些材料进行基因分型，这些引物与先前鉴定的棍棒病抗性基因相关。用TASSEL 5.0筛选后，保留68661个SNP标记，用于评价4个一般线性模型（GLM）和4个混合线性模型（MLM）。PCA + K和Q + K MLM模型在分位数-分位数图中给出了观测值与预期分布的最小偏差，因此用于SNP-clubroot关联分析。此外，利用pcr标记得到的108个等位基因和表型数据，采用PCA + K模型进行分析。鉴定出45个snp和4个基于pcr的标记与13种病原菌（2F、3H、5I、6M、8N、2B、3A、30、5C、5G、5K、5L和8P）的耐药性密切相关。这些标记显示芜菁染色体A03的上、下段和A08的中间段是与不同磷抗性相关的基因组热点。brassicaepathotypes。
Rutabaga [Brassica napusssp.napobrassica(L.) Hanelt] is reported to be an excellent source of clubroot (Plasmodiophora brassicae) resistance genes. In this study, 124 rutabaga accessions from the Nordic countries (Norway, Sweden, Finland, Denmark, and Iceland) were evaluated for their reaction to five single-spore isolates representingP. brassicaepathotypes 2F, 3H, 5I, 6M, and 8N and 12 field isolates representing pathotypes 2B, 3A, 3O, 5C, 5G, 5K, 5L, 5X (two isolates, L-G2 and L-G3), 8E, 8J, and 8P. The accessions were also genotyped using a 15K Brassica SNP array and 60 PCR-based primers linked to previously identified clubroot resistance genes. Six thousand eight hundred sixty-one SNP markers were retained after filtering with TASSEL 5.0, and used to evaluate four general linear models (GLM) and four mixed linear models (MLM). The PCA + K and Q + K MLM models gave the minimal deviance of the observed from the expected distribution in quantile-quantile plots, and hence were used for SNP-clubroot association analyses. In addition, 108 alleles derived from the PCR-based markers and the phenotypic data were analyzed with the PCA + K model. Forty-five SNPs and four PCR-based markers were identified to be associated strongly with resistance to isolates representing 13 pathotypes (2F, 3H, 5I, 6M, 8N, 2B, 3A, 3O, 5C, 5G, 5K, 5L, and 8P). These markers revealed the top and bottom segments of rutabaga chromosome A03 and the middle segment of chromosome A08 as genomic hotspots associated with resistance to the differentP. brassicaepathotypes.