Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility
Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility
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DOI：
10.1016/j.devcel.2012.05.007
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发表时间：
2012-07-17
影响因子：
11.8
通讯作者：
Raz, Erez
中科院分区：
文献类型：
--
作者：
Goudarzi, Mehdi;Banisch, Torsten U.;Raz, Erez
单细胞迁移是发育、稳态和疾病的关键过程。然而，对引导细胞进入体内运动行为的基本细胞机制的控制在很大程度上是未知的。在这里，我们报告了一组最小参数的识别，这些参数的调节赋予适当的形态和细胞运动性。通过敲除 Dead end（miRNA 功能的负调节因子），斑马鱼原始生殖细胞变得不动，被用作识别恢复运动过程的平台。我们将肌球蛋白收缩性、细胞粘附性和皮质特性定义为适当调节足以恢复该细胞类型的细胞迁移的因素。通过 miRNA-430 功能和 RNA 结合蛋白 Dead end 的作用之间的平衡来实现对这些细胞特征水平的严格控制，有效地将不动的原始生殖细胞转化为相对于环境中的细胞主动迁移的极化细胞。
Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment.