Detection of lfrA and tap efflux pump genes among clinical isolates of non-pigmented rapidly growing mycobacteria
Detection of lfrA and tap efflux pump genes among clinical isolates of non-pigmented rapidly growing mycobacteria
复制标题
DOI：
10.1016/j.ijantimicag.2009.06.026
复制
发表时间：
2009-11-01
影响因子：
10.8
通讯作者：
Fernandez-Roblas, R.
中科院分区：
文献类型：
--
作者：
Esteban, J.;Martin-de-Hijas, N. Z.;Fernandez-Roblas, R.
本研究旨在检测临床分离的非色素快速生长分枝杆菌（NPRGM）的LfrA和Tap外排泵。基因检测采用聚合酶链反应（PCR），每个基因设计特异性引物。采用微量肉汤稀释参比法分析菌株对多西环素、替加环素和环丙沙星的敏感性。共纳入166株临床分离株。在4株（2.4%）菌株中检出lfrA基因，其中龟分枝杆菌2株（占该菌种的6.7%）、福图分枝杆菌1株（占1.1%）和粘原分枝杆菌1株（占14.3%）。在109株（65.7%）菌株中检出tap基因，其中脓肿分枝杆菌3株（33.3%）、龟分枝杆菌12株（40%）、福图分枝杆菌75株（84.3%）、马格里特分枝杆菌2株（40%）、潜行分枝杆菌15株（68.2%）、肺泡分枝杆菌1株和猪分枝杆菌1株；粘液原分枝杆菌未检出阳性。阳性菌株和阴性菌株对强力霉素的耐药性无差异（Fisher精确检验，P = 0.055）。lfrA在NPRGM的临床分离株中罕见，而tap则更为常见。未发现外排泵的存在与对喹诺酮类或四环素类药物的耐药性之间存在相关性。(C) 2009 Elsevier B.V.与International Society of Chemotherapy。版权所有。
This study was performed to detect LfrA and Tap efflux pumps among clinical isolates of non-pigmented rapidly growingmycobacteria (NPRGM). Gene detection was performed using polymerase chain reaction (PCR) with specific primers designed for each gene. Susceptibility of the strains to doxycycline, tigecycline and ciprofloxacin was analysed using the broth microdilution reference technique. In total, 166 clinical isolates were included in the study. The lfrA gene was detected in four strains (2.4%), comprising two strains of Mycobacterium chelonae (6.7% of this species), one Mycobacterium fortuitum (1.1%) and one Mycobacterium mucogenicum (14.3%). The tap gene was detected in 109 strains (65.7%), comprising 3 Mycobacterium abscessus (33.3%), 12 M. chelonae (40%), 75 M. fortuitum (84.3%), 2 Mycobacterium mageritense (40%), 15 Mycobacterium peregrinum (68.2%), 1 Mycobacterium alvei and 1 Mycobacterium porcinum; no strains of M. mucogenicum were tap-positive. No differences between tap-positive and negative strains were observed for resistance to doxycycline (Fisher's exact test, P = 0.055). lfrA is rare among clinical isolates of NPRGM, whilst tap is found more commonly. No correlation was detected between the presence of the efflux pumps and resistance to quinolones or tetracyclines. (C) 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.