Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes.
Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes.
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猪PLIN和M6PRBP1基因的克隆、染色体定位及表达模式
DOI：
10.1186/1297-9686-40-2-215
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发表时间：
2008-03
期刊：
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
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作者：
Tao X;Jihong Y;Li G;Bin F;Yi Z;Xiaodong C;Peichao Z;Yang Z
PAT蛋白以三个PLIN/ADRP/TIP47 （PAT）蛋白命名，其中PLIN代表perilipin， ADRP代表脂肪分化相关蛋白，TIP47代表47 kDa的尾部相互作用蛋白，现已正式命名为M6PRBP1代表甘露糖-6-磷酸受体结合蛋白1，是细胞内脂滴结合蛋白。它们位于包裹脂滴的外膜单层，参与细胞内脂质的代谢。本文介绍了猪PLIN和M6PRBP1基因的克隆和测序，这两个基因的染色体定位，以及猪PAT基因的表达模式。序列分析表明，猪PLIN cDNA包含一个1551 bp的开放阅读框，编码516个氨基酸；猪M6PRBP1 cDNA包含一个1320 bp的编码区，编码439个氨基酸。比较不同物种间的PLIN和M6PRBP1氨基酸序列，发现猪和牛的蛋白最为保守。通过IMpRH面板的辐射杂交分析，猪PLIN和M6PRBP1基因分别定位到猪染色体7和2上。对猪的表达分析显示，脂肪组织中高表达PLIN mRNA，小肠、肾脏和脾脏中高表达M6PRBP1 mRNA，脂肪组织、肺和脾脏中高表达ADRP mRNA。
The PAT proteins, named after the three PLIN/ADRP/TIP47 (PAT) proteins, PLIN for perilipin, ADRP for adipose differentiation-related protein and TIP47 for tail-interacting protein of 47 kDa, now officially named M6PRBP1 for mannose-6-phosphate receptor binding protein 1, is a set of intracellular lipid droplet binding proteins. They are localized in the outer membrane monolayer enveloping lipid droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine PLIN and M6PRBP1 cDNAs, the chromosome mapping of these two genes, as well as the expression pattern of porcine PAT genes. Sequence analysis shows that the porcine PLIN cDNA contains an open reading frame of 1551 bp encoding 516 amino acids and that the porcine M6PRBP1 cDNA contains a coding region of 1320 bp encoding 439 amino acids. Comparison of PLIN and M6PRBP1 amino-acid sequences among various species reveals that porcine and bovine proteins are the most conserved. Porcine PLIN and M6PRBP1 genes have been mapped to pig chromosomes 7 and 2, respectively, by radiation hybrid analysis using the IMpRH panel. Expression analyses in pig showed a high expression of PLIN mRNA in adipose tissue, M6PRBP1 mRNA in small intestine, kidney and spleen and ADRP mRNA in adipose tissue, lung and spleen.