A Practical Protocol for the Conditional Depletion of Rho Isoforms in Human Embryonic Stem Cells.
A Practical Protocol for the Conditional Depletion of Rho Isoforms in Human Embryonic Stem Cells.
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人胚胎干细胞中 Rho 同种型条件性消耗的实用方案。
DOI：
10.1007/978-1-4939-8612-5_20
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发表时间：
2018
影响因子：
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通讯作者：
Ohgushi Masatoshi
中科院分区：
文献类型：
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作者：
鮎川友紀;八月朔日泰和;山崎正和;Ohgushi Masatoshi
人类多能干细胞在培养中无限增殖和存活，同时保持基因组完整性，为研究人类分子生物学提供了独特的机会。在这里，我们介绍了一种基于RNA干扰的人类胚胎干细胞诱导基因沉默方案，该方案在操作简单/方便、成本/时间性能和适用性方面具有几个优势。利用这种方法，我们成功地阐明了rho家族小gtpase在人胚胎干细胞中的独特作用。
Human pluripotent stem cells indefinitely proliferate and survive in culture while retaining genomic integrity, providing a unique opportunity to study human molecular biology. Here, we introduced an RNA interference-based protocol of inducible gene silencing in human embryonic stem cells, which has several advantages in handling simplicity/convenience, cost/time performance, and applicability. Using this method, we had succeeded to elucidate the isoform-unique roles of Rho-family small GTPases in human embryonic stem cells.