Isolation and characterization of neurofilaments from mammalian brain.
Isolation and characterization of neurofilaments from mammalian brain.
复制标题
哺乳动物大脑中神经丝的分离和表征。
DOI：
10.1021/bi00504a028
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
WilliamsJr,RC
中科院分区：
文献类型：
--
作者：
Runge,MS;Schlaepfer,WW;WilliamsJr,RC
马沙尔S作者：William W. Schlaepfer，§ and Robley C.小威廉姆斯 *摘要：本文介绍了一种从哺乳动物脑中大量快速分离神经丝的方法，并提出了与周围神经的神经丝相同的证据。采用差速离心结合Bio-Gel A-150 m凝胶过滤的方法从牛脑和大鼠脑的高速培养上清液中纯化神经丝。十二烷基硫酸钠凝胶电泳检测其蛋白质组成。牛脑神经丝的主要多肽分子量分别为210000、160000、69000和55000;大鼠脑神经丝的主要多肽分子量分别为200000、145000、68000和55000。每个物种中最大的三种多肽似乎对应于作为神经丝组分的蛋白质的“三联体”[Lasek，R. J.，&霍夫曼，P. N. 03 The Dog of the Dog（1976）
Marschall S. Runge,* William W. Schlaepfer, § and Robley C. Williams, Jr.* abstract: A procedure is described for rapid isolation of neurofilaments in large quantity from mammalian brain, and evidence is presented to establish their identity with the neu-rofilaments of peripheral nerve. Neurofilaments were purified from high-speed supernatants of bovine and rat brain by differential centrifugation combined with gel filtration on Bio-Gel A-150m. Their protein composition was examined by sodium dodecyl sulfate gel electrophoresis. The major polypeptides of neurofilaments from bovine brain had mo-lecular weights of 210000, 160000, 69000, and 55000, while those of neurofilaments from rat brain had molecular weights of 200000, 145 000, 68 000, and 55 000. The largest three of these polypeptidesin each species appearto correspond to the “triplet” of proteins that are components of neurofilaments [Lasek, R. J., & Hoffman, P. N.(1976) Cold Spring Harbor