Silencing of dehydrin CaDHN1 diminishes tolerance to multiple abiotic stresses in Capsicum annuum L.
Silencing of dehydrin CaDHN1 diminishes tolerance to multiple abiotic stresses in Capsicum annuum L.
复制标题
沉默脱水蛋白 CaDHN1 会降低辣椒对多种非生物胁迫的耐受性。
DOI：
10.1007/s00299-015-1862-1
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发表时间：
2015-12-01
影响因子：
6.2
通讯作者：
Gong, Zhen-Hui
中科院分区：
文献类型：
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作者：
Chen, Ru-gang;Jing, Hua;Gong, Zhen-Hui
我们从辣椒中克隆了一个脱水蛋白基因CaDHN1，在低温、盐胁迫、渗透胁迫和水杨酸(SA)处理下，CaDHN1的表达显著上调。脱水蛋白(DHNS)是第二组晚期胚胎发生丰富(LEA)蛋白的一个亚家族，被认为在提高植物的非生物胁迫耐受性方面发挥着重要作用。本研究从辣椒品种P70(Capsicum annuum L.)的6~8个真叶苗中获得了一个完整的表达序列标签。在我们实验室旁边。然而，辣椒中的dhn基因还没有得到很好的描述。根据这个EST序列，我们克隆了一个dhn基因，命名为CaDHN1，并研究了该基因在不同胁迫下的反应和表达。结果表明，CaDHN1具有DHN特异性保守的K-区和S区，编码219个氨基酸。系统进化分析表明，CaDHN1属于SKN亚组。组织表达谱分析表明，Cadh N1主要在果实和花中表达。冷、盐、渗透胁迫和水杨酸(SA)处理显著上调CaDHN1的表达，而脱落酸(ABA)和重金属处理对CaDHN1表达无明显影响。利用病毒诱导的基因沉默(VIGS)技术使CaDHN1功能丧失，导致对冷、盐和渗透胁迫的耐受性降低。综上所述，这些结果表明CaDHN1在辣椒植株的非生物逆境抗性调节中起着重要作用。
We cloned a dehydrins gene CaDHN1 from pepper and the expression of CaDHN1 was markedly upregulated by cold, salt, osmotic stresses and salicylic acid (SA) treatment.Dehydrins (DHNs) are a subfamily of group 2 late embryogenesis-abundant (LEA) proteins that are thought to play an important role in enhancing abiotic stress tolerance in plants. In this study, a DHN EST (Expressed Sequence Tag) was obtained from 6 to 8 true leaves seedlings of pepper cv P70 (Capsicum annuum L.) by our laboratory. However, the DHN gene in pepper was not well characterized. According to this EST sequence, we isolated a DHN gene, designated as CaDHN1, and investigated the response and expression of this gene under various stresses. Our results indicated that CaDHN1 has the DHN-specific and conserved K- and S- domain and encodes 219 amino acids. Phylogenetic analysis showed that CaDHN1 belonged to the SKn subgroup. Tissue expression profile analysis revealed that CaDH N1 was expressed predominantly in fruits and flowers. The expression of CaDHN1 was markedly upregulated in response to cold, salt, osmotic stresses and salicylic acid (SA) treatment, but no significant change by abscisic acid (ABA) and heavy metals treatment. Loss of function of CaDHN1 using the virus-induced gene silencing (VIGS) technique led to decreased tolerance to cold-, salt- and osmotic-induced stresses. Overall, these results suggest that CaDHN1 plays an important role in regulating the abiotic stress resistance in pepper plants.