itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2.
itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2.
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itk，一种由白细胞介素 2 诱导的 T 细胞特异性酪氨酸激酶基因。
DOI：
10.1073/pnas.89.23.11194
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发表时间：
1992
影响因子：
11.1
通讯作者：
Stephen Desiderio
中科院分区：
文献类型：
--
作者：
Janet D Siliciano;Theresa A. Morrow;Stephen Desiderio
T淋巴细胞通过与抗原、淋巴因子和细胞粘附分子的相互作用而被激活。酪氨酸磷酸化在这些途径的信号传导中都很重要，但除了与CD4和CD8相关的Src家族成员p56lck外，所涉及的蛋白酪氨酸激酶尚未确定。我们在这里描述了一个酪氨酸激酶基因，我们已经命名为itk （il -2诱导的t细胞激酶）。itk基因指定一个72kda蛋白酪氨酸激酶，该激酶与Src家族成员相关，但缺乏Src激酶的两个特征：n端肉豆肉酰化共识序列和C端附近的调节酪氨酸残基。对小鼠组织和细胞系的分析表明，itk在T细胞谱系中特异性表达，这表明itk编码的酪氨酸激酶在T淋巴细胞特有的信号转导途径中起作用。将IL-2添加到应答性T细胞中，itk RNA与IL-2R α平行增加，暗示itk参与T细胞活化。
T lymphocytes are activated by interactions with antigens, lymphokines, and cell adhesion molecules. Tyrosine phosphorylation has been implicated as important in signaling through each of these pathways, but except for p56lck, a member of the Src family that associates with CD4 and CD8, the protein-tyrosine kinases involved have not been defined. We describe here a tyrosine kinase gene that we have designated itk (for IL-2-inducible T-cell kinase). The itk gene specifies a 72-kDa protein-tyrosine kinase that is related to members of the Src family but lacks two features characteristic of Src kinases: an N-terminal myristoylation consensus sequence and a regulatory tyrosine residue near the C terminus. Analysis of mouse tissues and cell lines indicates that itk is specifically expressed in the T-cell lineage, suggesting that the tyrosine kinase encoded by itk functions in a signal transduction pathway unique to T lymphocytes. On addition of IL-2 to responsive T cells, itk RNA increases in parallel with that of IL-2R alpha, implicating itk in T-cell activation.