Copper biosorption by chemically treated Micrococcus luteus cells
Copper biosorption by chemically treated Micrococcus luteus cells
复制标题
化学处理的藤黄微球菌细胞对铜的生物吸附
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
H. Kamada
中科院分区：
文献类型：
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作者：
A. Nakajima;M. Yasuda;H. Yokoyama;H. Ohya‐Nishiguchi;H. Kamada
In order to clarify the binding states of copper in microbial cells, copper biosorption from aqueous systems using the chemically treated Micrococcus luteus IAM 1056 cells (hot water-treated, diluted NaOH-treated, chloroform–methanol-treated, and chloroform–methanol/concentrated KOH-treated cells) was examined. The intact cells of M. luteus adsorbed 527 μmol of copper per g cells, and its copper adsorption was very rapid and was affected by the solution pH. The chloroform–methanol/concentrated KOH-treated cells showed higher copper biosorption capacity than the intact and the other chemically treated cells. The electron paramagnetic resonance (EPR) parameters, g∥ and |A∥|, of Cu(II) ion in microbial cells indicate that Cu(II) ion in the intact and all the chemically treated cells have coordination environments with nitrogen and oxygen as donor atoms, being similar to those of type II proteins. The parameter g∥ also indicated that the coupling between Cu(II) ion and the cell materials in the CHCl3–MeOH/concentrated KOH-treated cells is rather more stable than those between Cu(II) ion and the cell materials in the other treated cells.
