A high-content screening platform utilizing polarization anisotropy and FLIM microscopy
A high-content screening platform utilizing polarization anisotropy and FLIM microscopy
复制标题
利用偏振各向异性和 FLIM 显微镜的高内涵筛选平台
DOI：
--
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
M. Irving
中科院分区：
文献类型：
--
作者：
D. Matthews;S. Ameer;P. Barber;G. Pierce;R. Newman;B. Vojnovic;L. Carlin;M. Keppler;T. Ng;K. Suhling;M. Irving
研制了一种全自动高含量筛选显微镜，利用荧光各向异性成像和荧光寿命显微镜来鉴定药物筛选试验中eGFP和mRPF1之间的Förster共振能量转移。宽视场偏振分辨成像仪用于同时捕获eGFP和mRFP1荧光发射的平行和垂直分量，以提供受体退极化的高速测量。供体激发态寿命测量执行使用激光扫描显微镜，然后用于确定FRET效率在一个特定的分析。使用突变Jurkat人t细胞进行原理验证试验，以说明FRET首先被鉴定然后由我们的高含量筛选系统量化的过程。
An automated high-content screening microscope has been developed which uses fluorescence anisotropy imaging and fluorescence lifetime microscopy to identify Förster resonant energy transfer between eGFP and mRPF1 in drug screening assays. A wide-field polarization resolved imager is used to simultaneously capture the parallel and perpendicular components of both eGFP and mRFP1 fluorescence emission to provide a high-speed measurement of acceptor depolarization. Donor excited state lifetime measurements performed using laser scanning microscopy is then used to determine the FRET efficiency in a particular assay. A proof-of-principle assay is performed using mutant Jurkat human T-cells to illustrate the process by which FRET is first identified and then quantified by our high-content screening system.
影响因子：
3.4
作者：
Mattheyses, AL;Hoppe, AD;Axelrod, D
通讯作者：
Axelrod, D
使用β1整合素缺陷的人T细胞来鉴定对整合素功能至关重要的β1整合素胞质结构域序列。
影响因子：
3.3
作者：
Romzek,NC;Harris,ES;Dell,CL;Skronek,J;Hasse,E;Reynolds,PJ;Hunt3rd,SW;Shimizu,Y
通讯作者：
Shimizu,Y
影响因子：
3.4
作者：
Rizzo, MA;Piston, DW
通讯作者：
Piston, DW