Correspondence of Giemsa C-band with DAPI/CMA Fluorochrome Staining Pattern in Aconitum sanyoense (Ranunculaceae)

Correspondence of Giemsa C-band with DAPI/CMA Fluorochrome Staining Pattern in Aconitum sanyoense (Ranunculaceae)
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山乌头(毛茛科)Giemsa C 带与 DAPI/CMA 荧光染料染色模式的对应关系

DOI:
10.1508/cytologia.56.135
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发表时间:
1991
期刊:
影响因子:
1
通讯作者:
H. Okada
H. Okada
中科院分区:
生物学4区
文献类型:
--
作者:
H. Okada

文献摘要

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本文研究了同一标本的Giemsa C-带与DAPI/CMA荧光染料带型的对应性。结果表明,存在多种C带,即富AT(C+/DAPI+/CMA-)、富GC(C+/DAPI-/CMA+)和中间(C+/DAPI-/CMA-)C带。此外,一些所谓的常染色质片段显示富含AT的DNA序列(C-/DAPI+/CMA-)。这些带型的组合的确定可能会提前详细检测该物种的个体之间的细胞遗传学变异。
Correspondence of banding patterns of Giemsa C-band and DAPI/CMA fluorochromes of the same specimen of Aconitum sanyoense was examined. It appeared that there were various C-bands, that is, AT-rich (C+/DAPI+/CMA-), GC-rich (C+/DAPI-/CMA+), and intermediate (C+/DAPI-/CMA-) C-bands. Furthermore, some so-called euchromatic segments showed AT-rich DNA sequences (C-/DAPI+/CMA-). Determination of the combination of these banding patterns may advance detailed detection of cytogenetical variation among individuals of this species.