Microworm optode sensors limit particle diffusion to enable in vivo measurements
Microworm optode sensors limit particle diffusion to enable in vivo measurements
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DOI：
10.1073/pnas.1015544108
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发表时间：
2011-02-15
影响因子：
11.1
通讯作者：
Clark, Heather A.
中科院分区：
文献类型：
--
作者：
Ozaydin-Ince, Gozde;Dubach, J. Matthew;Clark, Heather A.
已经有各种各样的纳米颗粒被用于体内用途，从基因和药物递送到肿瘤成像和生理监测。使用纳米粒子来测量生理状况，同时通过皮肤进行荧光处理，这为微创健康监测提供了一种理想的方法。在这里，我们创造了独特的颗粒，具有所有必要的物理特性，作为体内报告，但从注射点扩散最小化。这些被称为微虫的颗粒具有涂覆有生物相容性多孔膜的圆柱形形状，该生物相容性多孔膜具有大的表面积与体积比，同时保持大的流体动力学半径。我们使用这些微蠕虫来创建荧光钠传感器，用于皮下注射后的体内钠浓度检测器。然而，微虫的概念有可能扩展到其他类型的聚合物的固定化，用于连续的生理检测或分子的传递。
There have been a variety of nanoparticles created for in vivo uses ranging from gene and drug delivery to tumor imaging and physiological monitoring. The use of nanoparticles to measure physiological conditions while being fluorescently addressed through the skin provides an ideal method toward minimally invasive health monitoring. Here we create unique particles that have all the necessary physical characteristics to serve as in vivo reporters, but with minimized diffusion from the point of injection. These particles, called microworms, have a cylindrical shape coated with a biocompatible porous membrane that possesses a large surface-area-to-volume ratio while maintaining a large hydrodynamic radius. We use these microworms to create fluorescent sodium sensors for use as in vivo sodium concentration detectors after subcutaneous injection. However, the microworm concept has the potential to extend to the immobilization of other types of polymers for continuous physiological detection or delivery of molecules.