Anxiety in rats with bile duct ligation is associated with activation of JNK3 mitogen-activated protein kinase in the hippocampus
Anxiety in rats with bile duct ligation is associated with activation of JNK3 mitogen-activated protein kinase in the hippocampus
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DOI：
10.1007/s11011-020-00542-1
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发表时间：
2020-02-12
影响因子：
3.6
通讯作者：
Khaledi, Shiler
中科院分区：
文献类型：
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作者：
Ahmadi, Shamseddin;Khaledi, Shiler
我们研究了胆管结扎（BDL）大鼠的焦虑样行为及其与海马JNK3和P38 MAPKs表达的关系。雄性Wistar大鼠接受假手术或BDL作为肝硬化HE大鼠模型。在BDL的第1天和第7、14、21、28天，使用手术前2小时的光/暗盒试验来确定焦虑样行为。在BDL的第28天，分别用qPCR和western blotting方法检测海马JNK3和p38基因和蛋白的表达水平。结果显示，肝硬化HE模型大鼠在BDL的28天内焦虑增加。分子数据显示，与假手术对照组相比，肝硬化HE模型大鼠海马组织中Jnk3基因表达、Jnk3蛋白及磷酸化Jnk3蛋白水平均显著升高。然而，与假手术对照组相比，肝硬化HE模型大鼠海马组织中p38和磷酸化p38的基因表达和蛋白水平均无明显变化。我们得出结论，海马中JNK3 MAPK表达和激活的增加可能是肝硬化HE大鼠焦虑样行为的基础，至少是部分原因。
We examine the anxiety-like behaviors in rats with bile duct ligation (BDL), as well as its relationship with the expression of JNK3 and P38 MAPKs in rat hippocampus. Male Wistar rats undergo either sham operation or BDL as a rat model of cirrhotic HE. The anxiety-like behaviors are determined using a light/dark box test two hours befor the surgery on day 1 and on days 7, 14, 21 and 28 of BDL. The gene and protein expression levels of JNK3 and p38 in the hippocampus were examined respectively with qPCR and western blotting methods on day 28 of BDL. The results revealed that anxiety was increased in the cirrhotic HE model rats during 28 days of BDL. The molecular data indicated that the gene expression of Jnk3 and protein levels of JNK3, as well as phospho-JNK3, significantly increased in the hippocampus of the cirrhotic HE model rats compared to the sham control group. However, the results revealed no significant changes in the gene expression and the protein levels of p38 as well as phospho-p38 in the hippocampus of the cirrhotic HE model rats compared to the sham control group. We conclude that the increases in the expression and activation of JNK3 MAPK in the hippocampus may underlie, at least partly, the anxiety-like behaviors in rats with cirrhotic HE.