Effects of glutamine supplementation on the immune status in weaning piglets with intrauterine growth retardation
Effects of glutamine supplementation on the immune status in weaning piglets with intrauterine growth retardation
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DOI：
10.1080/1745039x.2012.683325
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发表时间：
2012-09
影响因子：
2
通讯作者：
X. Zhong;Wei Li;Xuexin Huang;Yuanxiao Wang;Lili Zhang;Yanmin Zhou;A. Hussain;Tian Wang
中科院分区：
文献类型：
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作者：
X. Zhong;Wei Li;Xuexin Huang;Yuanxiao Wang;Lili Zhang;Yanmin Zhou;A. Hussain;Tian Wang
胎儿宫内发育迟缓(IUGR)新生儿常伴有细胞免疫功能低下，断奶可能进一步加重IUGR对免疫系统发育和功能的不良影响。在本研究中，我们研究了补充谷氨酰胺对IUGR断奶仔猪肠道免疫状态的影响，重点研究了免疫反应改变的分子机制。21日龄断奶IUGR仔猪，每隔12h口服每公斤体重1.22g丙氨酸或1g谷氨酰胺，通过补充谷氨酰胺提高断奶仔猪的增重和肠道重量。添加谷氨酰胺的仔猪血清免疫球蛋白水平显著高于对照仔猪。补充谷氨酰胺可降低血清和空肠中IL-1和IL-8的产生，增加血清中IL-4和空肠中IL-4和IL-10的浓度。补充谷氨酰胺后，空肠热休克蛋白70(HSP70)的表达增加，但抑制物κB的降解和核因子-κB(NF-κB)活性降低。综上所述，补充谷氨酰胺可增强IUGR断奶仔猪的免疫应答。谷氨酰胺在宫内发育迟缓中的作用与上调HSP70表达和抑制NF-κB活化有关。
Neonates with intrauterine growth retardation (IUGR) often suffer from impaired cellular immunity, and weaning may further aggravate adverse effects of IUGR on development and function of the immune system. In this study, we investigated effects of glutamine supplementation on immune status in the intestines of weaning pigs with IUGR, focusing on molecular mechanisms underlying altered immune response. Piglets with IUGR were weaned at 21 days of age and received orally 1.22 g alanine or 1 g glutamine per kg body weight every 12 h. Weight gain and intestinal weight of weaning piglets were increased by glutamine supplementation. Levels of serum IgG in piglets supplemented with glutamine were increased compared with Control piglets. The production of IL-1 and IL-8 in the serum and jejunum was decreased by glutamine supplementation, whereas the levels of IL-4 in the serum and the concentrations of IL-4 and IL-10 in the jejunum were increased. The expression of heat shock protein 70 (Hsp70) in the jejunum was increased by glutamine supplementation, but the degradation of inhibitor κB and the activity of nuclear factor-κB (NF-κB) were decreased. In conclusion, glutamine supplementation enhanced immune response in weaning piglets with IUGR. The effects of glutamine in IUGR are associated with increased Hsp70 expression and suppression of NF-κB activation.