Global proteome analysis links lysine acetylation to diverse functions in Oryza sativa
Global proteome analysis links lysine acetylation to diverse functions in Oryza sativa
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全球蛋白质组分析将赖氨酸乙酰化与水稻的多种功能联系起来
DOI：
10.1002/pmic.201700036
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发表时间：
--
期刊：
影响因子：
3.4
通讯作者：
Zhiyun Gong
中科院分区：
文献类型：
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作者：
Chao Xue;Shuai Liu;Chen Chen;Jun Zhu;Xibin Yang;Yong Zhou;Rui Guo;Zhiyun Gong
赖氨酸乙酰化（Kac）是真核生物和原核生物中重要的蛋白质翻译后修饰。在此，我们报告的全球蛋白质组分析的结果Kac及其在水稻（水稻）的不同功能。我们在水稻幼苗的866个蛋白质中鉴定了1353个Kac位点。共有11个Kac基序是保守的，45%的已鉴定蛋白质定位于叶绿体。在所有乙酰化蛋白质中，38个Kac位点组合在核心组蛋白中。生物信息学分析表明，Kac发生在涉及各种生物过程，特别是光合作用的各种蛋白质上。所鉴定的蛋白质的蛋白质-蛋白质相互作用网络提供了Kac有助于广泛的调节功能的进一步证据。此外，我们证明了组蛋白H3（赖氨酸27和36）的乙酰化水平在冷胁迫下增加。综上所述，我们的方法全面地描述了Kac在水稻生长发育中的调控作用。
Lysine acetylation (Kac) is an important protein post‐translational modification in both eukaryotes and prokaryotes. Herein, we report the results of a global proteome analysis of Kac and its diverse functions in rice (Oryza sativa). We identified 1353 Kac sites in 866 proteins in rice seedlings. A total of 11 Kac motifs are conserved, and 45% of the identified proteins are localized to the chloroplast. Among all acetylated proteins, 38 Kac sites are combined in core histones. Bioinformatics analysis revealed that Kac occurs on a diverse range of proteins involved in a wide variety of biological processes, especially photosynthesis. Protein–protein interaction networks of the identified proteins provided further evidence that Kac contributes to a wide range of regulatory functions. Furthermore, we demonstrated that the acetylation level of histone H3 (lysine 27 and 36) is increased in response to cold stress. In summary, our approach comprehensively profiles the regulatory roles of Kac in the growth and development of rice.