The Increased Ratio of Blood CD56bright NK to CD56dim NK Is a Distinguishing Feature of Primary Sjogren's Syndrome
The Increased Ratio of Blood CD56bright NK to CD56dim NK Is a Distinguishing Feature of Primary Sjogren's Syndrome
复制标题
血液 CD56(bright) NK 与 CD56(dim) NK 比率增加是原发性干燥综合征的一个显着特征
DOI：
10.1155/2020/7523914
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发表时间：
2020-07-09
影响因子：
4.1
通讯作者：
Dong, Lingli
中科院分区：
文献类型：
--
作者：
Ming, Bingxia;Wu, Tong;Dong, Lingli
目标。本研究的目的是表征pSS患者循环CD56(+)NK细胞亚群及其在pSS患者诊断和/或预测预后中的潜在价值。我们纳入了52例符合2002年AECG标准或2012年ACR标准的pSS患者和20名年龄和性别匹配的健康志愿者。流式细胞术检测外周血标本中NK细胞和CD56 NK细胞亚群的频率和绝对数量。其他实验室参数如IgG水平和补体蛋白水平从临床系统中提取。与健康对照组相比，pSS患者外周血NK细胞的频率和绝对数量均有所减少。CD56（亮）NK细胞亚群比例升高，而CD56（暗）NK细胞亚群比例降低，导致pSS患者CD56（亮）NK与CD56（暗）NK之比显著升高。ROC分析显示，CD56（亮）NK/CD56（暗）NK比值AUC为0.838，pSS患者的最佳诊断分界点为0.0487。CD56（亮）NK/CD56（暗）NK比值与IgG水平正相关，与补体蛋白C3和C4水平负相关。更重要的是，在其他自身免疫性疾病如SLE和igg4相关疾病中，CD56（亮）/CD56（暗）NK比值或轻微升高或无变化。我们的研究结果表明，血液CD56（亮）NK与CD56（暗）NK的比例可能对pSS具有相对特异性的诊断价值。
Objective. The aim of this study was to characterize the subsets of circulating CD56(+)NK cells in pSS patients and their potential value in the diagnosis and/or prediction of prognosis in patients with pSS.Methods. We included 52 pSS patients fulfilling the 2002 AECG criteria or 2012 ACR criteria and 20 age- and gender-matched healthy volunteers. The frequency and absolute number of NK cells and CD56 NK cell subsets in peripheral blood samples were detected by flow cytometry. Other laboratory parameters such as the IgG level and complement protein levels were extracted from the clinical system.Results. Both the frequency and the absolute number of peripheral blood NK cells were reduced in pSS patients compared to healthy controls. The proportion of CD56(bright)NK cell subset was increased, and the proportion of CD56(dim)NK cell subset was decreased among NK cells, resulting in the ratio of CD56(bright)NK to CD56(dim)NK which was significantly elevated in pSS patients. ROC analysis indicated that the AUC of CD56(bright)NK/CD56(dim)NK ratio was 0.838, and the best diagnostic cut-off point was 0.0487 for pSS patients. Furthermore, this CD56(bright)NK/CD56(dim)NK ratio was positively correlated with the IgG level and negatively correlated with the complement protein C3 and C4 levels. More importantly, the CD56(bright)/CD56(dim)NK ratio was either slightly increased or not changed in other autoimmune diseases such as SLE and IgG4-related disease.Conclusion. Our findings suggest that the ratio of blood CD56(bright)NK to CD56(dim)NK might have a diagnostic value relatively specific for pSS.