Protein–DNA and protein–hormone interactions in prealbumin: a model of the thyroid hormone nuclear receptor?
Protein–DNA and protein–hormone interactions in prealbumin: a model of the thyroid hormone nuclear receptor?
复制标题
前白蛋白中蛋白质-DNA 和蛋白质-激素相互作用：甲状腺激素核受体模型？
作者：
C. Blake;S. Oatley
对激素结合蛋白前白蛋白的高分辨率x射线分析表明，它与双螺旋DNA具有结构互补性。所提出的结合位点由两个对称相关的β-片组成，其中含有一对螺旋排列的臂，可以与DNA宽槽中的碱基相互作用。回文靶序列由蛋白质的对称性表示。前白蛋白上的两个相同的甲状腺激素结合位点位于一个完全贯穿分子的通道中。这两个结构特征表明前白蛋白是甲状腺激素核受体的模型，提供了一些详细的预测其性质。
High resolution X-ray analysis of the hormone-binding protein prealbumin has shown that it has a structural complementarity to double-helical DNA. The proposed binding site is composed of two symmetry-related β-sheets containing a pair of helically disposed arms, which can interact with the bases in the wide groove of DNA. A palindromic target sequence is indicated by the symmetry of the protein. The two identical thyroid hormone binding sites on prealbumin are located in a channel that runs completely through the molecule. These two structural features suggest prealbumin as a model for the thyroid hormone nuclear receptor, providing a number of detailed predictions of its properties.