Perception of the pathogen-induced peptide RGF7 by the receptor-like kinases RGI4 and RGI5 triggers innate immunity in Arabidopsis thaliana.
Perception of the pathogen-induced peptide RGF7 by the receptor-like kinases RGI4 and RGI5 triggers innate immunity in Arabidopsis thaliana.
复制标题
受体样激酶 RGI4 和 RGI5 对病原体诱导的肽 RGF7 的感知会触发拟南芥的先天免疫。
DOI：
10.1111/nph.17197
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Xiangzong Meng
中科院分区：
文献类型：
--
作者：
Xiaoyang Wang;Na Zhang;Li;Yunxia He;Chao Cai;Jinggeng Zhou;Jia Li;Xiangzong Meng
信号肽在植物生长、发育和防御中发挥着至关重要的作用。我们在此报道拟南芥分泌肽根分生生长因子 7 (RGF7) 作为内源激发子来触发植物免疫。在细菌病原体丁香假单胞菌感染后，拟南芥叶片中 RGF7 前体编码基因 (preRGF7) 的表达被高度诱导。病原体响应性 preRGF7 表达受转录因子 WRKY33 及其上游蛋白激酶 MPK3/MPK6 和 CPK5/CPK6 调节。在没有病原体攻击的情况下，转基因拟南芥植物中化学诱导的 preRGF7 表达足以引发免疫反应。转基因拟南芥中 preRGF7 表达的预诱导也导致免疫反应增强和对丁香假单胞菌感染的抵抗力增加。生化和遗传分析表明，RGF7 可以被叶表达的 RGF1 INSENSITIVE (RGI) 家族受体 RGI4 和 RGI5 感知。体细胞胚胎发生受体激酶 (SERK) BAK1 和 SERK4 也通过与 RGI4 和 RGI5 形成 RGF7 诱导的受体复合物来参与 RGF7 感知。这些结果表明，由 RGI4/RGI5-BAK1/SERK4 受体复合物感知的病原体诱导的 RGF7 肽充当新的损伤相关分子模式 (DAMP)，并在拟南芥免疫中发挥重要作用。
Signaling peptides play crucial roles in plant growth, development and defense. We report here that the Arabidopsis thaliana secreted peptide, ROOT MERISTEM GROWTH FACTOR 7 (RGF7), functions as an endogenous elicitor to trigger plant immunity. Expression of the RGF7 precursor-encoding gene (preRGF7) is highly induced in Arabidopsis leaves upon infection by the bacterial pathogen Pseudomonas syringae. The pathogen-responsive preRGF7 expression is regulated by the transcription factor WRKY33 and its upstream protein kinases MPK3/MPK6 and CPK5/CPK6. In the absence of pathogen attack, chemically induced expression of preRGF7 in transgenic Arabidopsis plants was sufficient to trigger immune responses. Pre-induction of preRGF7 expression in transgenic Arabidopsis also led to enhanced immune responses and increased resistance to P. syringae infection. Biochemical and genetic analyses demonstrated that RGF7 is perceived by the leaf-expressed RGF1 INSENSITIVE (RGI) family receptors RGI4 and RGI5. The SOMATIC EMBRYOGENESIS RECEPTOR KINASES (SERKs) BAK1 and SERK4 are also involved in RGF7 perception via forming RGF7-induced receptor-complexes with RGI4 and RGI5. These results indicate that the pathogen-induced RGF7 peptide, perceived by the RGI4/RGI5-BAK1/SERK4 receptor complexes, acts as a new damage-associated molecular pattern (DAMP) and plays a significant role in Arabidopsis immunity.