Complete mitogenome sequence of the jewel beetle, Chrysochroa fulgidissima (Coleoptera: Buprestidae)
Complete mitogenome sequence of the jewel beetle, Chrysochroa fulgidissima (Coleoptera: Buprestidae)
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DOI：
10.1080/19401730802644978
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发表时间：
2009-01-01
影响因子：
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通讯作者：
Kim, Iksoo
中科院分区：
文献类型：
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作者：
Hong, Mee Yeon;Jeong, Heon Cheon;Kim, Iksoo
在这项研究中，我们确定了完整的线粒体基因组的宝石甲虫，Chrysochroafulgidissima（鞘翅目：天牛科），从四个重叠的片段。C. fulgidissima有丝分裂基因组显示出与昆虫中最常见类型相同的基因排列和内容。C. COI基因是一个不寻常的基因，没有典型的ATN密码子。875 bp的A + T-丰富的区域是最短的鞘翅目有丝分裂基因组，迄今已被测序的全部。基因组最不寻常的特征是在富含A + T的区域内存在三个tRNA样序列：两个tRNA（Leu）（UUR）样序列和一个tRNA（Asn）样序列。这些序列段具有适当的反密码子序列和形成二级结构的潜力，但在茎中也存在许多错配。系统发育分析中使用的蛋白质编码基因的13个氨基酸序列之间的串联的鞘翅目总科（Buprestoidea和Elateroidea属于下Elateriformia，和Escheromeloidea和Tenebrioroidea属于下Cucujiformia）的贝叶斯推断，最大似然分析，最大简约分析显示缺乏强有力的支持单系Elateriformia。
In this study, we determined the complete mitochondrial genome of the jewel beetle, Chrysochroa fulgidissima (Coleoptera: Buprestidae), from four overlapping fragments. The 15,592 bp C. fulgidissima mitogenome exhibits a gene arrangement and content identical to the most common type in insects. The start codon of the C. fulgidissima COI gene is unusual, in that no typical ATN codon is available. The 875 bp A + T-rich region is the shortest among the coleopteran mitogenomes that have thus far been sequenced in their entirety. The most unusual feature of the genome is the presence of three tRNA-like sequences within the A + T-rich region: two tRNA(Leu) (UUR)-like sequences and one tRNA(Asn)-like sequence. These sequence stretches have the proper anticodon sequence and the potential to form secondary structures, but also harbor many mismatches in the stems. Phylogenetic analysis using a concatenation of 13 amino acid sequences of protein-coding genes among the available sequenced species of coleopteran superfamilies (Buprestoidea and Elateroidea belonging to the infraorder Elateriformia, and Chrysomeloidea and Tenebrioroidea belonging to the infraorder Cucujiformia) by Bayesian inference, maximum-likelihood analysis, and maximum-parsimony analysis revealed a lack of strong support for monophyletic Elateriformia.