PacC in the nematophagous fungus Clonostachys rosea controls virulence to nematodes.
PacC in the nematophagous fungus Clonostachys rosea controls virulence to nematodes.
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DOI：
10.1111/j.1462-2920.2010.02191.x
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发表时间：
2010-07
影响因子：
5.1
通讯作者：
C. Zou;Hui-Hui Tu-Hui;Xian-Yong Liu;N. Tao;Ke-Qin Zhang
中科院分区：
文献类型：
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作者：
C. Zou;Hui-Hui Tu-Hui;Xian-Yong Liu;N. Tao;Ke-Qin Zhang
食线虫真菌是植物和动物寄生线虫的常用生防制剂。然而，相对知之甚少的环境属性赋予这些真菌的致病性。在这份报告中，我们调查的作用PacC介导的pH值的反应，在发病机制中的食草真菌Clonostachys rosea。我们从该真菌中鉴定了一个pacC直向同源物，并发现其转录本在C. rosea在其感染线虫的早期阶段。pacC的破坏导致在碱性pH下生长减慢，改变了孢子形成，减少了分生孢子和减弱了对线虫的毒力。细胞外丝氨酸蛋白酶PrC，一个假定的毒力因子，表达下调的pacC突变体。PrC转录水平显着高于碱性生长条件下比酸性生长条件下。启动子活性分析和电泳迁移率变动分析表明，pH通过PacC途径对PrC的调控发生在转录水平。因此，PacC在C.玫瑰色。
Nematophagous fungi are commonly used as biological control agents of plant and animal parasitic nematodes. However, relatively little is known of the environmental attributes conferring pathogenicity in these fungi. In this report, we investigated the role of PacC-mediated pH response in the pathogenesis of the nematophagous fungus Clonostachys rosea. We identified a pacC orthologue from this fungus and found that its transcript was elevated in C. rosea during the early stage of its infection of nematode. Disruption of pacC resulted in slowed growth at alkaline pH, altered filamentation, reduced conidiation and attenuated virulence to nematodes. The expression of an extracellular serine protease PrC, a putative virulence factor, was downregulated in the pacC mutants. The PrC transcript levels were significantly higher under alkaline growth conditions than under acidic growth conditions. Promoter activity analysis and electrophoretic mobility shift assay indicated that the regulation of PrC by pH via the PacC pathway occurred at the transcriptional level. In conclusion, PacC functions as a positive regulator of virulence to nematodes in C. rosea.