CRYOCRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES - A GENERALLY APPLICABLE METHOD
CRYOCRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES - A GENERALLY APPLICABLE METHOD
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DOI：
10.1107/s0108768187008632
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发表时间：
1988-02-01
影响因子：
1.9
通讯作者：
HOPE, H
中科院分区：
文献类型：
--
作者：
HOPE, H
已经开发了允许在低温（接近液氮）温度下对生物大分子进行简易X射线数据收集的方法。首先将晶体从其母液转移到烃环境中，然后用标准玻璃纤维（无毛细管）固定，并在衍射装置上用冷氮气流原位快速冷却。这种方法可以防止晶体中的溶剂冻结，从而保持晶体的完整性。从低温数据收集可以导致分辨率的显著提高，并且在数据收集的持续时间内，冷却晶体中的辐射损伤被大大减少或消除。
Methods have been developed that allow facile X-ray data collection for biological macromolecules at cryogenic (near liquid N2) temperatures. The crystals are first transferred from their mother liquor to a hydrocarbon environment, then mounted with a standard glass fiber (no capillary), and flash cooled in situ with a cold nitrogen stream on the diffraction apparatus. This approach prevents freezing of the solvent in the crystals, so that they maintain their crystallographic integrity. Significant improvement of resolution can result from the cryogenic data collection, and radiation damage in the cooled crystals is greatly reduced, or eliminated, for the duration of data collection.