Psychophysical measurement of contrast sensitivity in the behaving mouse
Psychophysical measurement of contrast sensitivity in the behaving mouse
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DOI：
10.1152/jn.00609.2011
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发表时间：
2012-02-01
影响因子：
2.5
通讯作者：
Maunsell, John H. R.
中科院分区：
文献类型：
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作者：
Histed, Mark H.;Carvalho, Lauren A.;Maunsell, John H. R.
Histed MH，Carvalho LA，Maunsell JH.行为小鼠对比敏感度的心理物理测量。J Neurophysiol 107：758-765，2012.首次发表于2011年11月2日; doi：10.1152/jn.00609.2011.-为了了解哺乳动物神经回路中的活动如何控制行为，由于遗传学、光学和生理学方法的融合，小鼠是一个很有前途的模型系统。精确控制和量化行为的能力对这些研究也至关重要。我们开发了一个操作性视觉检测范式，使视觉心理物理测量：头部固定的小鼠通过按下一个杠杆的反应。我们设计这项任务是为了对大脑皮层的行为进行神经生理学研究，大脑皮层的活动在不同的试验中是可变的，神经元同时编码多种类型的信息。为了研究面对这种复杂性的神经反应，我们训练小鼠做一项任务，每天进行数百次试验，可以测量感知阈值。我们使用这个任务来测量行为小鼠的视觉敏锐度和最小可检测对比度。我们发现小鼠的对比度反应函数在形状上与其他物种相似。它们可以检测低对比度刺激，峰值对比度阈值为2%，相当于类似于人类视觉中15度偏心。小鼠敏锐度适中，上限接近0.5周/度，与先前数据一致。
Histed MH, Carvalho LA, Maunsell JH. Psychophysical measurement of contrast sensitivity in the behaving mouse. J Neurophysiol 107: 758-765, 2012. First published November 2, 2011; doi:10.1152/jn.00609.2011.-To understand how activity in mammalian neural circuits controls behavior, the mouse is a promising model system due to the convergence of genetic, optical, and physiological methods. The ability to control and quantify behavior precisely is also essential for these studies. We developed an operant visual detection paradigm to make visual psychophysical measurements: head-fixed mice make responses by pressing a lever. We designed this task to permit neurophysiological studies of behavior in cerebral cortex, where activity is variable from trial to trial and neurons encode many types of information simultaneously. To study neural responses in the face of this complexity, we trained mice to do a task where they perform hundreds of trials daily and perceptual thresholds can be measured. We used this task to measure both visual acuity and the minimum detectable contrast in behaving mice. We found that the mouse contrast response function is similar in shape to other species. They can detect low-contrast stimuli, with a peak contrast threshold of 2%, equivalent to similar to 15 degrees eccentric in human vision. Mouse acuity is modest, with an upper limit near 0.5 cycles/degrees, consistent with prior data.