Low integrated DNA repair score and lung cancer risk.
Low integrated DNA repair score and lung cancer risk.
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低综合 DNA 修复评分与肺癌风险。
DOI：
10.1158/1940-6207.capr-13-0318
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Livneh,Zvi
中科院分区：
文献类型：
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作者：
Sevilya,Ziv;Leitner-Dagan,Yael;Pinchev,Mila;Kremer,Ran;Elinger,Dalia;Rennert,HedyS;Schechtman,Edna;Freedman,LaurenceS;Rennert,Gad;Paz-Elizur,Tamar;Livneh,Zvi
DNA修复是防止DNA损伤、突变和癌症的主要机制。采用功能方法，我们研究了综合DNA修复评分与肺癌风险的关系，该评分通过外周血单个核细胞中的三种酶DNA修复活性来测量。该小组包括AP内切酶1 (APE1)， 8-氧鸟嘌呤DNA糖基化酶（OGG1）和甲基嘌呤DNA糖基化酶（MPG）的检测，所有这些都可以修复DNA氧化损伤，作为碱基切除修复途径的一部分。本研究对96例肺癌患者和96例对照患者进行了一项基于人群的盲法病例对照研究，对照组按性别、年龄（±1岁）、居住地和种族（犹太人/非犹太人）匹配。测量三种DNA修复活性，并计算每个个体的综合DNA修复OMA （OGG1，MPG和ape1）评分。条件logistic回归分析显示，综合DNA修复OMA评分最低的个体与最高的个体相比，患肺癌的风险增加，OR = 9.7；95%置信区间（CI）， 3.1-29.8；P< 0.001, or = 5.6；95% ci, 2.1-15.1；交叉验证后P< 0.001。这些结果表明，这种危险因素的DNA修复面板可能对肺癌风险评估有用，有助于预防和通过低剂量计算机断层扫描等技术进行早期检测。癌症预防；7 (4);398 - 406。AACR©2013。
DNA repair is a prime mechanism for preventing DNA damage, mutation, and cancers. Adopting a functional approach, we examined the association with lung cancer risk of an integrated DNA repair score, measured by a panel of three enzymatic DNA repair activities in peripheral blood mononuclear cells. The panel included assays for AP endonuclease 1 (APE1), 8-oxoguanine DNA glycosylase (OGG1), and methylpurine DNA glycosylase (MPG), all of which repair oxidative DNA damage as part of the base excision repair pathways. A blinded population-based case–control study was conducted with 96 patients with lung cancer and 96 control subjects matched by gender, age (±1 year), place of residence, and ethnic group (Jews/non-Jews). The three DNA repair activities were measured, and an integrated DNA repair OMA (OGG1,MPG, andAPE1) score was calculated for each individual. Conditional logistic regression analysis revealed that individuals in the lowest tertile of the integrated DNA repair OMA score had an increased risk of lung cancer compared with the highest tertile, with OR = 9.7; 95% confidence interval (CI), 3.1–29.8;P< 0.001, or OR = 5.6; 95% CI, 2.1–15.1;P< 0.001 after cross-validation. These results suggest that pending validation, this DNA repair panel of risk factors may be useful for lung cancer risk assessment, assisting prevention and referral to early detection by technologies such as low-dose computed tomography scanning.Cancer Prev Res; 7(4); 398–406. ©2013 AACR.