Differences between single- and dual-species biofilms of Streptococcus mutans and Veillonella parvula in growth, acidogenicity and susceptibility to chlorhexidine
Differences between single- and dual-species biofilms of Streptococcus mutans and Veillonella parvula in growth, acidogenicity and susceptibility to chlorhexidine
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DOI：
10.1111/j.1600-0722.2006.00262.x
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发表时间：
2006-02-01
影响因子：
1.9
通讯作者：
ten Cate, JM
中科院分区：
文献类型：
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作者：
Kara, D;Luppens, SBI;ten Cate, JM
变形链球菌被认为是龋齿的主要病原体，它在牙菌斑（一种多物种生物膜）中大量繁殖。变形链球菌和小韦荣球菌之间的代谢相互作用已被提出。在这项研究中，我们建立了一个生物膜模型来量化单物种（变形链球菌或小韦荣球菌）和双物种（变形链球菌和小韦荣球菌）生物膜的形成，并且我们确定了各自生物膜在生长、酸形成以及对氯己定的反应方面的差异。使用聚苯乙烯96孔微量滴定板来形成生物膜。这些生物膜暴露于不同浓度的氯己定（0.025 - 0.4毫克/毫升）和处理条件下。通过活菌计数来评估生物膜的生长以及氯己定的效果。72小时后，所有生物膜类型中两种菌的生存能力相似（约为每孔10⁸个菌落形成单位）。在48小时和72小时时，双物种生物膜中的乳酸积累显著低于变形链球菌单物种生物膜。当包括中和步骤时，双物种生物膜比单物种生物膜对氯己定的敏感性更低。这些结果表明，双物种生物膜中的细菌与单物种生物膜中的细菌具有不同的特性。
Streptococcus mutans, considered a primary pathogen in dental caries, thrives in dental plaque, which is a multispecies biofilm. Metabolic interactions between S. mutans and Veillonella parvula have been suggested. In this study we developed a biofilm model to quantify single-species (S. mutans or V. parvula) and dual-species (S. mutans and V. parvula) biofilm formation, and we identified the differences between the respective biofilms in terms of growth, acid formation, and response to chlorhexidine. Polystyrene 96-well microtiter plates were used for biofilm formation. These biofilms were exposed to various chlorhexidine concentrations (0.025-0.4 mg ml(-1)) and treatment conditions. Growth of the biofilms and the effects of chlorhexidine were evaluated by viable counts. Viability of the two species in all biofilm types was similar (approximate to 10(8) colony-forming units per well) after 72 h. Lactic acid accumulation of dual-species biofilms was significantly lower at 48 and 72 h than single-species biofilms of S. mutans. Dual-species biofilms were less susceptible to chlorhexidine than single-species biofilms when a neutralization step was included. These results indicate that bacteria in dual-species biofilms have different properties from bacteria in single-species biofilms.