HISTAMINE TURNOVER IN RAT-BRAIN
HISTAMINE TURNOVER IN RAT-BRAIN
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DOI：
10.1016/0006-8993(74)90929-9
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
DISMUKES, K;SNYDER, SH
通过两种独立的技术测量组胺在大鼠脑中的半衰期。静脉注射组氨酸脱羧酶抑制剂（溴甲酚或α-肼基组氨酸）可使下丘脑和丘脑-中脑的组胺迅速减少20-25%。根据消耗曲线的斜率，估计这些区域的组胺半衰期约为30秒。开发了一种方法，以估计组胺半衰期的时间过程中的[3 H]组胺出现后，脑室内注射[3 H]组氨酸。使用这种方法，我们的数据表明下丘脑组胺的半衰期不超过约1分钟，比身体其他部位的值短得多，其中组胺周转需要数小时或数周。通过比较新合成的[3 H]组胺与内源性组胺的减少程度，确定了所检查的脑区域中的所有组胺显然都位于该快速翻转池内。
The half-life of histamine in the rat brain was measured by two independent techniques. Intravenous injection of inhibitors of histidine decar☐ylase (brocresine or α-hydrazinohistidine) produced a prompt 20–25% depletion of histamine in hypothalamus and thalamus-midbrain. From the slope of the depletion curves, histamine in these regions was estimated to have a half-life of about 30 sec. A method was developed to estimate histamine half-life from the time course of [3H]histamine appearance following intraventricular injection of [3H]histidine. With this method, our data indicate a half-life for hypothalamic histamine of no more than about 1 min, much shorter than values in other parts of the body, where histamine turnover takes hours or weeks. By comparing the extent of reduction of newly synthesized [3H]histamine with that of endogenous histamine, it was determined that all histamine in the brain regions examined apparently lies within this rapidly turning over pool.