A stereological study of synapse number in the epileptic human hippocampus
A stereological study of synapse number in the epileptic human hippocampus
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DOI：
10.3389/fnana.2011.00008
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发表时间：
2011-02-24
影响因子：
2.9
通讯作者：
DeFelipe, Javier
中科院分区：
文献类型：
--
作者：
Alonso-Nanclares, Lidia;Kastanauskaite, Asta;DeFelipe, Javier
海马硬化是顽固性颞叶癫痫（TLE）患者切除内侧颞结构最常见的病理。在这里，我们使用立体学方法比较了从TLE患者手术切除的非硬化和硬化海马组织之间突触和神经元的总体密度。具体地说，我们检查了耻骨下和CA1可能发生的变化，这些区域似乎对这些患者癫痫发作的发展和/或维持至关重要。我们发现硬化CA1的突触和神经元密度显著下降，而硬化海马的下托突触密度没有变化，但神经元密度更高。由于硬化海马的枕骨下显示出神经元密度的显著增加，以及其他各种神经化学变化，我们认为，表面上正常的硬化海马枕骨下在分子和突触水平上的神经元回路发生了深刻的改变，这可能对癫痫活动的发展或维持至关重要。
Hippocampal sclerosis is the most frequent pathology encountered in resected mesial temporal structures from patients with intractable temporal lobe epilepsy (TLE). Here, we have used stereological methods to compare the overall density of synapses and neurons between nonsclerotic and sclerotic hippocampal tissue obtained by surgical resection from patients with TLE. Specifically, we examined the possible changes in the subiculum and CA1, regions that seem to be critical for the development and/or maintenance of seizures in these patients. We found a remarkable decrease in synaptic and neuronal density in the sclerotic CA1, and while the subiculum from the sclerotic hippocampus did not display changes in synaptic density, the neuronal density was higher. Since the subiculum from the sclerotic hippocampus displays a significant increase in neuronal density, as well as a various other neurochemical changes, we propose that the apparently normal subiculum from the sclerotic hippocampus suffers profound alterations in neuronal circuits at both the molecular and synaptic level that are likely to be critical for the development or maintenance of seizure activity.