Tip-enhanced Raman Spectroscopy of Lipid Bilayers in Water with an Alumina- and Silver-coated Tungsten Tip
Tip-enhanced Raman Spectroscopy of Lipid Bilayers in Water with an Alumina- and Silver-coated Tungsten Tip
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DOI：
10.2116/analsci.29.865
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发表时间：
2013-09
影响因子：
1.6
通讯作者：
Atsushi Nakata;Tomonori Nomoto;T. Toyota;M. Fujinami
中科院分区：
文献类型：
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作者：
Atsushi Nakata;Tomonori Nomoto;T. Toyota;M. Fujinami
研究了水溶液中负载磷脂双层膜的针尖增强拉曼光谱。检测了两种双层膜：1，2-二棕榈酰- sn -甘油-3-磷酸胆碱（DPPC）和1，2-二油酰- sn -甘油-3-磷酸胆碱（DOPC）.我们制造的氧化铝和银涂层钨针尖在水中非常坚固。DOPC双层膜的TERS光谱具有很大的时间依赖性，而DPPC双层膜的TERS光谱在探针针尖下没有观察到这种时间依赖性。DOPC双层膜的光谱变化的液晶相的流动性进行了讨论。因此，时间分辨TERS具有表征流体磷脂双层膜中的不均匀性和扩散的潜力。
Tip-enhanced Raman spectroscopy (TERS) of supported phospholipid bilayers in an aqueous environment is discussed in this paper. Two bilayer membranes were examined: 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC). We fabricated alumina- and silver-coated tungsten tips that are very robust in water. There was a large time-dependence in the TERS spectra for the DOPC bilayers, whereas no such time-dependence was observed in the DPPC bilayer spectra under the probe tip. The spectral changes of DOPC bilayers are discussed in terms of the fluidity of the liquid crystalline phase. Time-resolved TERS thus has the potential to characterize inhomogeneity and diffusion in fluidic phospholipid bilayer membranes.