Alterations in Deciduous Dental Pulp Cells Cultured with Serum-free Medium
Alterations in Deciduous Dental Pulp Cells Cultured with Serum-free Medium
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DOI：
10.2485/jhtb.24.17
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Arita, Kenji
中科院分区：
文献类型：
--
作者：
Harada, Kyoko;Kawai, Saki;Arita, Kenji
人骨髓间充质干细胞能够分化为各种类型的细胞，在再生医学中的应用非常有用。以往的研究表明，可以从牙髓中分离出干细胞，而从乳牙中剥离的牙髓因其较高的增殖率而成为一种有用的组织工程选择。对于临床应用来说，有必要进行细胞的体外培养，并尽快获得足够数量的细胞。此外，有必要确认体外培养的细胞的变化，以确保它们具有合适的特性。本研究利用基因芯片技术分析了无血清培养的人乳牙髓细胞的增殖、细胞形态和基因表达的变化。我们发现，在无血清培养的STK2中培养的细胞具有较高的细胞增殖率，并且细胞在细胞形态上倾向于相互紧密联系。此外，在STK2培养的乳牙牙髓细胞中，3248个基因的表达水平比含胎牛血清的培养液高2倍。
Human mesenchymal stem cells are capable of differentiating into various cell types and are useful for applications in regenerative medicine. Previous studies have indicated that stem cells can be isolated from dental pulp, and dental pulp exfoliated from deciduous teeth has become a useful alternative for dental tissue engineering because of its higher proliferation rate. For clinical application, it is necessary to culture cells in vitro and to obtain sufficient numbers of cells as quickly as possible. Furthermore, it is necessary to confirm the change in cells cultured in vitro in order to ensure that they have suitable characteristics. In this study, to investigate the changes in human deciduous dental pulp cells cultured with serum-free media, we analyzed cell proliferation, cell morphology and gene expression changes by microarray analysis. We found a high cell proliferation rate in cells that were cultured in STK2 that is a serum-free medium, and cells tended to remain in close contact with one another in cell morphology. In addition, 3248 genes were expressed at >2-fold higher levels in dental pulp cells from deciduous teeth cultured with STK2 compared with medium containing fetal bovine serum for 3 days.