Effects of fixation and enzymatic digestion on the immunohistochemical demonstration of laminin and fibronectin in paraffin embedded tissue.
Effects of fixation and enzymatic digestion on the immunohistochemical demonstration of laminin and fibronectin in paraffin embedded tissue.
复制标题
固定和酶消化对石蜡包埋组织中层粘连蛋白和纤连蛋白免疫组织化学显示的影响。
DOI：
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发表时间：
1984
影响因子：
3.4
通讯作者：
A. D'ardenne
中科院分区：
文献类型：
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作者：
P. Kirkpatrick;A. D'ardenne
比较了不同固定剂和酶消化程序对石蜡包埋组织中纤连蛋白和层粘连蛋白免疫组织化学显示的影响。所测试的固定剂均无法在不进行酶消化的情况下对这些蛋白质进行染色。在固定或新鲜冷冻组织中均未发现胞质内层粘连蛋白。甲醛乙酸中的固定对于纤连蛋白的展示并不令人满意；在甲醛升华物中长时间固定对于层粘连蛋白的展示并不令人满意。在常规 10% 福尔马林盐水中固定后可获得最佳结果。除非在乙醇乙酸中固定，否则胰蛋白酶对于揭开层粘连蛋白抗原完全无效；它对于显示纤连蛋白抗原仅部分有效。最好的结果是通过蛋白酶消化获得的，但胃蛋白酶是一种足够的替代品，尽管可靠性稍差。这些酶的使用浓度可能低于通常推荐的浓度。
The effects of different fixatives and enzymatic digestion procedures on the immunohistochemical demonstration of fibronectin and laminin in paraffin embedded tissues have been compared. None of the fixatives tested enabled staining of these proteins without enzymatic digestion. No intracytoplasmic laminin was found either in fixed or in fresh frozen tissue. Fixation in formol acetic acid was unsatisfactory for demonstration of fibronectin; prolonged fixation in formol sublimate was unsatisfactory for demonstration of laminin. Optimal results were achieved after fixation in routine 10% formol saline. Trypsin was completely ineffective for unmasking laminin antigens except after fixation in ethanol acetic acid; it was only partially effective for showing fibronectin antigens. The best results were obtained with protease digestion, but pepsin was an adequate, although slightly less reliable, alternative. These enzymes may be used at lower concentrations than usually recommended.