Neonatal Mortality, Vesicular Lesions and Lameness Associated with Senecavirus A in a US Sow Farm
Neonatal Mortality, Vesicular Lesions and Lameness Associated with Senecavirus A in a US Sow Farm
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DOI：
10.1111/tbed.12516
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发表时间：
2016-08-01
影响因子：
4.3
通讯作者：
Karriker, L.
中科院分区：
文献类型：
--
作者：
Canning, P.;Canon, A.;Karriker, L.
在美国，一项300头母猪从产仔到完成的猪手术中，新生仔猪的死亡率突然严重增加，发病率高，随后成年雌性和雄性猪的口鼻部和足部出现水泡性病变。将受影响的活仔猪提交进行诊断研究。样品经聚合酶链反应（PCR）检测，口蹄疫病毒、猪三角洲冠状病毒、猪流行性腹泻病毒、猪轮状病毒A型、B型和C型、传染性胃肠炎病毒和猪生殖与呼吸综合征病毒均为阴性。通过实时逆转录聚合酶链反应（rRT-PCR）从仔猪的血清、皮肤和粪便以及母猪的血清和粪便中检测到Senecavirus A（SV-A），以前称为Seneca Valley病毒。在细胞培养物中从仔猪样品中分离SV-A。猪组织中SV-A的VP 1基因序列测定也是成功的。进行了生物安保和疾病进入评价，查明了新病原体进入行动的潜在生物安保风险因素。这是美国首例将SV-A与严重但短暂的新生儿发病率和死亡率的临床过程相关联的病例报告，随后是繁殖动物中的水泡性病变。兽医和动物护理人员必须对水泡性外来动物疾病保持警惕，并向州动物卫生当局报告可疑的临床体征和病变，以便进行诊断检测和进一步调查。
A 300-sow farrow-to-finish swine operation in the United States experienced a sudden and severe increase in mortality in neonatal piglets with high morbidity followed by vesicular lesions on the snout and feet of adult females and males. Affected live piglets were submitted for diagnostic investigation. Samples tested polymerase chain reaction (PCR) negative for foot-and-mouth disease virus, porcine delta coronavirus, porcine epidemic diarrhoea virus, porcine rotavirus types A, B and C, transmissible gastroenteritis virus, and porcine reproductive and respiratory syndrome virus. Senecavirus A (SV-A) formerly known as Seneca Valley virus was detected by real-time reverse-transcription polymerase chain reaction (rRT-PCR) from serum, skin and faeces of piglets and from serum and faeces of sows. SV-A was isolated in cell culture from piglet samples. SV-A VP1 gene region sequencing from piglet tissues was also successful. A biosecurity and disease entry evaluation was conducted and identified potential biosecurity risks factors for the entry of new pathogens into the operation. This is the first case report in the United States associating SV-A with a clinical course of severe but transient neonatal morbidity and mortality followed by vesicular lesions in breeding stock animals. Veterinarians and animal caretakers must remain vigilant for vesicular foreign animal diseases and report suspicious clinical signs and lesions to state animal health authorities for diagnostic testing and further investigation.