MicroRNA-106b Regulates Milk Fat Metabolism via ATP Binding Cassette Subfamily A Member 1 (ABCA1) in Bovine Mammary Epithelial Cells
MicroRNA-106b Regulates Milk Fat Metabolism via ATP Binding Cassette Subfamily A Member 1 (ABCA1) in Bovine Mammary Epithelial Cells
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MicroRNA-106b 通过 ATP 结合盒亚家族 A 成员 1 (ABCA1) 调节牛乳腺上皮细胞中的乳脂代谢
DOI：
10.1021/acs.jafc.9b00622
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发表时间：
2019-04-10
影响因子：
6.1
通讯作者：
Yang, Zhangping
中科院分区：
文献类型：
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作者：
Chen, Zhi;Chu, Shuangfeng;Yang, Zhangping
研究调节奶牛乳脂合成的机制对于确定潜在的分子靶标至关重要，从长远来看，这些靶标可以帮助制定适当的分子育种计划。尽管一些研究表明，microRNA （miRNA）通过靶向特定基因来影响脂质代谢，但对牛乳腺组织中miRNA和靶mRNA数据的联合分析并未揭示控制乳脂合成的潜在机制。本研究的目的是利用高通量测序和生物信息学分析来鉴定miRNA和mRNA对，并进一步探讨它们在调节乳脂合成中的潜在作用。共检测到233对负相关的miRNA和mRNA对。其中，有162对mirna下调，靶mrna上调。在已鉴定的miRNA中，miR-106b可以结合ATP结合盒亚家族A成员1 （ABCA1）的3'-UTR，该基因先前被认为与牛乳脂肪合成呈正相关。miR-106b在牛乳腺上皮细胞中过表达导致甘油三酯和胆固醇含量降低，而抑制miR-106b使甘油三酯和胆固醇含量升高，证实了其在脂质代谢中的作用。本研究构建了miR-106b-ABCA1调控网络图谱，为靶向该基因在奶牛分子育种中的应用提供了理论依据。
Research on the mechanisms that regulate milk fat synthesis in dairy cows is essential to identify potential molecular targets that in the long term can help develop appropriate molecular breeding programs. Although some studies have revealed that microRNA (miRNA) affect lipid metabolism by targeting specific genes, joint analysis of miRNA and target mRNA data from bovine mammary tissue has revealed few clues regarding the underlying mechanisms controlling milk fat synthesis. The objective of the present study was to use high-throughput sequencing and bioinformatics analysis to identify miRNA and mRNA pairs and explore further their potential roles in regulating milk fat synthesis. A total of 233 pairs of negatively associated miRNA and mRNA pairs were detected. Among those, there were 162 pairs in which the miRNAs were down-regulated and the target mRNAs were up-regulated. Among the identified miRNA, miR-106b can bind the 3'-UTR of the ATP binding cassette subfamily A member 1 (ABCA1), a gene previously identified as having a positive association with bovine milk fat synthesis. The overexpression of miR-106b in bovine mammary epithelial cells caused a decrease in triglyceride and cholesterol content while the inhibition of miR-106b increased triglyceride and cholesterol content, confirming its role in lipid metabolism. The present study allowed for the construction of a miR-106b-ABCA1 regulatory network map, thus providing a theoretical basis to target this gene in the molecular breeding of dairy cows.