Olivocerebellar fiber maturation in normal and lurcher mutant mice: defective development in lurcher.
Olivocerebellar fiber maturation in normal and lurcher mutant mice: defective development in lurcher.
复制标题
正常和 lucher 突变小鼠的橄榄小脑纤维成熟：lucher 发育缺陷。
DOI：
10.1002/cne.902910308
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Eisenman,LM
中科院分区：
文献类型：
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作者：
Heckroth,JA;Goldowitz,D;Eisenman,LM
使用来自下橄榄的麦芽凝集素辣根过氧化物酶（WGA-HRP）的顺行运输，在出生后第 5 天（P5）和 P15 之间检查正常和 lucher 突变小鼠的橄榄小脑纤维成熟度。浦肯野细胞标记物 PEP-19 的免疫细胞化学用于证明相同材料中浦肯野细胞的发育。在突变动物和正常动物中，观察到区域发育差异，因此，在给定年龄进行比较时，小脑半球的皮层衬里的皮层似乎比小脑半球的皮层发育先进。在正常动物的初级裂中，第一个可识别的浦肯野细胞树突出现在P6，橄榄小脑纤维在P9首先进入其发育的攀爬阶段。在潜伏动物中，浦肯野细胞的发育按照这个时间表进行，但从未观察到橄榄小脑纤维进入分子层。这些传入细胞在浦肯野细胞周围维持密集的周巢，即使在 P13-15 潜伏者中也是如此。通过透射电子显微镜对 P14 luchers 的检查表明，橄榄小脑纤维在浦肯野细胞体细胞棘上形成突触，而篮细胞轴突未能在浦肯野细胞周围形成其典型的周巢。此外，可以观察到平行纤维在浦肯野细胞初级树突上的树突棘上形成突触。我们将这些结果解释为表明橄榄小脑纤维和浦肯野细胞树突之间的识别缺陷。对 lucher 的这种缺陷的分析可能揭示橄榄小脑纤维从周体到树突末端的正常转变是如何实现的。
Olivocerebellar fiber maturation was examined in normal and lurcher mutant mice between postnatal day 5 (P5) and P15, using the anterograde transport of wheat germ agglutinin‐horseradish peroxidase (WGA‐HRP) from the inferior olive. Immunocytochemistry for the Purkinje cell marker PEP‐19 was used to demonstrate Purkinje cell development in the same material. In mutant and normal animals, a regional developmental variation is observed such that, when compared at a given age, cortex lining the vermal fissures appears developmentally advanced over cortex in the cerebellar hemispheres. In the primary fissure of the normal animals, the first recognizable Purkinje cell dendrites appear on P6, and the olivocerebellar fibers first enter the climbing stage of their development on P9. In lurcher animals Purkinje cell development proceeds on this schedule, but olivocerebellar fibers are never observed to enter the molecular layer. These afferents maintain dense perisomatic nests around Purkinje cells, even in P13‐15 lurchers. Examination of P14 lurchers by transmission electron microscopy indicates that the olivocerebellar fibers form synapses on Purkinje cell somatic spines and that the basket cell axons fail to form their typical perisomal nests around Purkinje cells. In addition, parallel fibers can be observed to synapse on dendritic spines on the Purkinje cell primary dendrites. We interpret these results as indicating a recognition defect between olivocerebellar fibers and Purkinje cell dendrites. An analysis of this defect in lurcher may reveal how the normal transformation of olivocerebellar fibers, from perisomal to dendritic terminals, is achieved.