Effects of fatigue on depolarization- and caffeine-induced contractures of skinned fibres.
Effects of fatigue on depolarization- and caffeine-induced contractures of skinned fibres.
复制标题
疲劳对去极化和咖啡因引起的带皮纤维挛缩的影响。
DOI：
10.1046/j.0001-6772.2003.01243.x
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Williams,JH
中科院分区：
文献类型：
--
作者：
Williams,JH
Aims:Fatigue has been shown to cause intrinsic alterations in sarcoplasmic reticulum (SR) Ca2+release.Methods:In this investigation, frog semitendinosus muscles were stimulated to fatigue,in vitro(80 Hz, 100 ms, 1 train s−1, 5 min). Immediately after stimulation, single fibres were removed and skinned using either chemically or mechanically skinning. Contralateral muscle were treated similarly but were not stimulated.Results:In fatigued, saponin skinned fibres, contracture responses to low [caffeine] (4–8 mm) were depressed compared with control. However, responses to high concentrations (10–15 mm) were not different between conditions. In the fatigued, mechanically skinned fibres, responses to chloride depolarization were depressed at all [chloride] (20–100 mm) compared with control.Conclusions:These results suggest that fatigue causes intrinsic alterations in both the SR Ca2+release channel as well as communication between the transverse‐tubule and the SR.
