DNA-RAPD Fingerprinting and Cytogenetic Screening of Genotoxic and Antigenotoxic Effects of Aqueous Extracts of Costus Speciosus (Koen.)
DNA-RAPD Fingerprinting and Cytogenetic Screening of Genotoxic and Antigenotoxic Effects of Aqueous Extracts of Costus Speciosus (Koen.)
复制标题
DNA-RAPD 指纹图谱和细胞遗传学筛选 Costus Speciosus (Koen.) 水提取物的基因毒性和抗原毒性作用
DOI：
10.4197/sci.22-1.10
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Sameer H Qari
中科院分区：
文献类型：
--
作者：
Sameer H Qari
研究了传统植物Costus speciosus（Koen.）在沙特阿拉伯经常用于治疗各种疾病。用高浓度的Costus speciosus（Koen.）处理洋葱根尖分生组织，在48小时时取样。每种浓度（2、5、10、20 µg/mL），并进行细胞遗传学和分子遗传学试验（DNA-RAPD指纹图谱）。结果表明，该提取物无致染色体断裂或致突变活性，有丝分裂指数较对照组轻度降低。暴露时，所有浓度下的染色体畸变频率均无显著性。分裂细胞的畸变多为染色体畸变。RAPD结果显示，与对照相比，各处理的PCR电泳产物均为单态的遗传条带。同时也显示了C. speciosus作为抗遗传毒性和抗细胞毒性的潜力，对EMS诱导的DNA损伤，细胞毒性和染色体断裂的影响洋葱细胞。结果表明，C.结果表明，香葱提取物对洋葱细胞无明显的遗传毒性作用。此外，本研究还表明，联合治疗C。speciosus对EMS的遗传毒性作用有很强的抑制作用。这些结果有力地表明，Costus speciosus提取物没有遗传毒性，或细胞毒性，但可能是抗遗传毒性剂。
The present study was conducted to investigate the genotoxic and/or antigenotoxic effect of aqueous extract of the traditional plant Costus speciosus (Koen.) that is used frequently for the treatment of various disorders in Saudi Arabia. Root tip meristems of Allium cepa were treated with elevated concentrations of Costus speciosus (Koen.), Samples were taken at 48h. of each concentration (2, 5, 10, 20 µg/mL) and subjected to cytogenetic and molecular genetics assays (DNA-RAPD fingerprinting). It was found that the extract has no clastogenic or mutagenic activities, mitotic index decreased mildly compared with the control. Frequency of chromosomal aberrations showed no significance in all concentrations at time of exposure. Most aberrations of the dividing cells were disturbance of chromosomes. The RAPD results demonstrated monomorphic numbers of genetic bands mostly, which were the electrophoretic products of PCR for all treatments compared with the control. Also the results exhibited the ability of C. speciosus as antigenotoxic and anticytotoxic potential against EMS induced DNA damage , cytotoxic and clastogenic effects in Allium cepa cells. The presented data showed that C. speciosus extract could not induce significantly genotoxic effect on Allium cepa cells. Furthermore, this study implies that combined treatment of C. speciosus has a strong inhibitory role against the genotoxic action of EMS. These results strongly suggest that the extract of Costus speciosus is not genotoxic, or cytotoxic but might be anti-genotoxic agent.