Essential oils from Moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant Gram-negative bacteria
Essential oils from Moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant Gram-negative bacteria
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DOI：
10.1016/j.ijantimicag.2011.05.005
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发表时间：
2011-10-01
影响因子：
10.8
通讯作者：
Pages, Jean-Marie
中科院分区：
文献类型：
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作者：
Fadli, Mariam;Chevalier, Jacqueline;Pages, Jean-Marie
细菌耐药性是一个令人担忧的公共卫生问题。抗生素外排是细菌主要的非特异性耐药机制，外排泵参与多种重要革兰氏阴性病原菌的低水平敏感性。使用能够阻断细菌泵的分子是一种有吸引力的策略，但由于这些分子的局限性（稳定性、选择性、生物利用度、毒性等），一些研究报告仅部分有效。本研究的目的是寻找能够抑制耐药革兰氏阴性菌（大肠杆菌、产气肠杆菌、肺炎克雷伯菌、肠炎沙门氏菌血清型鼠伤寒杆菌和铜绿假单胞菌）外排泵系统的天然分子来源。结果表明，所研究的精油对所测试的细菌表现出有趣的活性。这种活性在外排泵抑制剂如苯丙氨酸精氨酸β -萘酰胺（PA β N）的存在下显著增强。脂多糖（LPS）结构在精油作用中的作用也在沙门氏菌脂多糖深粗突变体中得到了报道。此外，在低浓度（最低抑制浓度的一小部分）下使用的胸腺精油和布鲁索蒂胸腺精油能够显著增加几种耐药菌株对氯霉素的敏感性。这些结果表明，这些精油可以改变外排泵的活性，可能是开发新药的有吸引力的候选者，用于化疗致敏多药耐药菌株对临床使用的抗生素。(C) 2011 Elsevier B.V.与International Society of Chemotherapy。版权所有。
Bacterial drug resistance is a worrying public health problem. Antibiotic efflux is a major non-specific resistance mechanism used by bacteria, and efflux pumps are involved in the low-level susceptibility of various important Gram-negative pathogens. Use of molecules that can block bacterial pumps is an attractive strategy, but several studies report only partial efficacy owing to limits of these molecules (stability, selectivity, bioavailability, toxicity, etc.). The objective of this study was to search for natural sources of molecules able to inhibit efflux pump systems of resistant Gram-negative bacteria (Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Salmonella enterica serotype Typhimurium and Pseudomonas aeruginosa). The results indicate that the studied essential oils exhibit interesting activity against the tested bacteria. This activity was significantly enhanced in the presence of an efflux pump inhibitor such as phenylalanine arginyl beta-naphthylamide (PA beta N). The role of lipopolysaccharide (LPS) structure in the effect of essential oils was also reported in Salmonella LPS deep-rough mutants. In addition, essential oils of Thymus maroccanus and Thymus broussonetii, used at a low concentration (a fraction of the minimum inhibitory concentration), are able to significantly increase chloramphenicol susceptibility of several resistant isolates. These results demonstrate that these essential oils can alter efflux pump activity and may be attractive candidates to develop new drugs for chemosensitising multidrug-resistant strains to clinically used antibiotics. (C) 2011 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.