EFFECTS OF THERAPEUTIC ULTRASOUND ON JOINT MOBILITY AND COLLAGEN FIBRIL ARRANGEMENT IN THE ENDOMYSIUM OF IMMOBILIZED RAT SOLEUS MUSCLE
EFFECTS OF THERAPEUTIC ULTRASOUND ON JOINT MOBILITY AND COLLAGEN FIBRIL ARRANGEMENT IN THE ENDOMYSIUM OF IMMOBILIZED RAT SOLEUS MUSCLE
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DOI：
10.1016/j.ultrasmedbio.2008.09.001
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发表时间：
2009-02-01
影响因子：
2.9
通讯作者：
Yoshimura, Toshiro
中科院分区：
文献类型：
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作者：
Okita, Minoru;Nakano, Jiro;Yoshimura, Toshiro
本研究探讨治疗性超声波对固定大鼠比目鱼肌肌内膜胶原纤维排列及关节活动度的影响。22只雄性Wistar大鼠随机分为对照组（n = 5）和实验组（n = 17）。在实验组中，每只大鼠的双侧踝关节固定在秋季跖屈与石膏模型超过4周的时间。实验组中的5只动物在整个4周期间（制动组）被制动，而实验组中的其余大鼠被随机分为超声（US，n = 6）和假手术（n = 6）治疗组。在麻醉状态下，超声能量（频率1 MHz，强度1.0W/cm 2）持续作用于US组的小腿三头肌，15 min/d，6d/wk，制动4 wk。在假手术动物中，超声能量未输送至小腿三头肌;仅移动换能器头。踝关节活动度背屈在制动，假手术和US组显着小于对照组，而在US组，该参数显着大于在制动和假手术组。对照组和US组肌内膜中的胶原原纤维排列是纵向于肌纤维的轴;相反，在固定组和假手术组中是周向的。我们的研究结果表明，关节制动诱导关节活动度和肌内膜胶原纤维运动下降，此外，超声治疗可以防止这些变化。我们假设，在制动过程中的治疗性超声可以抑制恶化或肌肉挛缩。（电子邮件：mokita@nagasaki-u.ac.jp）（c）2009年世界医学和生物学超声联合会。
This study examined effects of therapeutic ultrasound oil joint mobility and collagen fibril arrangement ill the endomysium of immobilized rat soleus muscle. Twenty-two male Wistar rats were divided randomly into control (n = 5) and experimental groups (n = 17). In the experimental group, bilateral ankle joints of each rat were fixed in fall plantar flexion with a plaster cast over a 4-wk period. Five animals in the experimental group were immobilized throughout the 4-wk (immobilization group) period, whereas the remaining rats in the experimental group were randomly divided into the ultrasound (US, n = 6) and sham (n = 6) treatment groups. Under anesthesia, continuous ultrasonic energy (frequency, 1 MHz; intensity, 1.0 W/cm(2)) was delivered to the triceps surae muscle of the US group for 15 min per d, 6 d per wk over the 4-wk immobilization period. Ultrasonic energy was not delivered to the triceps surae muscle in sham animals; only the transducer head was moved. Ankle joint mobility on dorsiflexion in the immobilization, sham and US groups was significantly smaller than that of the control group, whereas in the US group, this parameter was significantly greater than in the immobilization and sham groups. Collagen fibril arrangement in the endomysium of the control and US groups was longitudinal to the axis of the muscle fibers; in contrast, it was circumferential in the immobilization and sham groups. Our findings revealed that joint immobilization induces decreased joint mobility and collagen fibril movement in the endomysium; furthermore, ultrasound treatment can prevent these changes. We hypothesized that therapeutic ultrasound during the immobilization process may inhibit deterioration or muscle contracture. (E-mail: mokita@nagasaki-u.ac.jp) (c) 2009 World Federation for Ultrasound in Medicine & Biology.