Terahertz imaging applied to cancer diagnosis
Terahertz imaging applied to cancer diagnosis
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DOI：
10.1088/0031-9155/55/16/001
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发表时间：
2010-08-21
影响因子：
3.5
通讯作者：
Eishii, Y.
中科院分区：
文献类型：
--
作者：
Brun, M-A;Formanek, F.;Eishii, Y.
我们报告太赫兹（THz）时域光谱成像的10 μ m厚的组织切片。根据标准病理学程序制备切片，并将其沉积在石英窗上用于反射几何学测量。可见光图像的同时采集使得能够配准THz图像，从而使用数字病理学工具来研究底层细胞结构和特定THz信息之间的联系。一个分析模型，考虑到太赫兹光束的偏振，它的入射角，参考和样品脉冲之间的光束移位，以及在样品内的多次反射来确定频率相关的复折射率。光谱图像通过使用聚类方法对提取的折射率数据进行分割而产生。可见光和太赫兹图像的比较表明，不仅在肿瘤和健康组织之间，而且在肿瘤内的光谱差异。使用主成分分析的进一步可视化表明不同的机制，图像对比度的起源。
We report on terahertz (THz) time-domain spectroscopy imaging of 10 mu m thick histological sections. The sections are prepared according to standard pathological procedures and deposited on a quartz window for measurements in reflection geometry. Simultaneous acquisition of visible images enables registration of THz images and thus the use of digital pathology tools to investigate the links between the underlying cellular structure and specific THz information. An analytic model taking into account the polarization of the THz beam, its incidence angle, the beam shift between the reference and sample pulses as well as multiple reflections within the sample is employed to determine the frequency-dependent complex refractive index. Spectral images are produced through segmentation of the extracted refractive index data using clustering methods. Comparisons of visible and THz images demonstrate spectral differences not only between tumor and healthy tissues but also within tumors. Further visualization using principal component analysis suggests different mechanisms as to the origin of image contrast.