Capillary responses to functional and pathological activations rely on the capillary states at rest
Capillary responses to functional and pathological activations rely on the capillary states at rest
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DOI：
10.1177/0271678x231156372
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发表时间：
2023-02
影响因子：
6.3
通讯作者：
Hiroki Suzuki;Hiroshi Takeda;H. Takuwa;Bin Ji;Makoto Higuchi;I. Kanno;K. Masamoto
中科院分区：
文献类型：
--
作者：
Hiroki Suzuki;Hiroshi Takeda;H. Takuwa;Bin Ji;Makoto Higuchi;I. Kanno;K. Masamoto
脑毛细血管在维持细胞活力，从而防止神经变性方面起着至关重要的作用。本研究的目的是基于三维微血管造影的大数据分析来表征静息和神经激活期间的脑毛细血管形态。神经血管反应进行了测量，使用遗传钙传感器在神经元和微血管造影与双光子显微镜，而神经活性调制刺激对侧胡须或癫痫发作引起的红藻氨酸。对于晶须刺激，84%的毛细血管部位没有显示出可检测的直径变化。其余10%和6%分别为扩张和收缩。毛细血管在扩张和收缩的位置之间的直径在休息时观察到显着差异。即使是癫痫发作也导致44%的毛细血管直径没有可检测到的变化，而56%的毛细血管扩张。扩张的程度取决于静止时的直径。总之，对脑毛细血管形态的大数据分析确定了至少两种类型的毛细血管状态：静止时直径相对较大且随时间推移而稳定的毛细血管，无论神经活动如何，以及静止时直径相对较小且根据神经活动而变化的毛细血管。
Brain capillaries play a crucial role in maintaining cellular viability and thus preventing neurodegeneration. The aim of this study was to characterize the brain capillary morphology at rest and during neural activation based on a big data analysis from three-dimensional microangiography. Neurovascular responses were measured using a genetic calcium sensor expressed in neurons and microangiography with two-photon microscopy, while neural acivity was modulated by stimulation of contralateral whiskers or by a seizure evoked by kainic acid. For whisker stimulation, 84% of the capillary sites showed no detectable diameter change. The remaining 10% and 6% were dilated and constricted, respectively. Significant differences were observed for capillaries in the diameter at rest between the locations of dilation and constriction. Even the seizures resulted in 44% of the capillaries having no detectable change in diameter, while 56% of the capillaries dilated. The extent of dilation was dependent on the diameter at rest. In conclusion, big data analysis on brain capillary morphology has identified at least two types of capillary states: capillaries with diameters that are relatively large at rest and stable over time regardless of neural activity and capillaries whose diameters are relatively small at rest and vary according to neural activity.