First microsatellite loci for spectacled salamanders (Salamandrina perspicillata and S. terdigitata) endemic to the Apennine peninsula

First microsatellite loci for spectacled salamanders (Salamandrina perspicillata and S. terdigitata) endemic to the Apennine peninsula
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DOI:
10.1007/s12686-011-9559-4
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发表时间:
2012-06-01
影响因子:
1.1
通讯作者:
Steinfartz, Sebastian
Steinfartz, Sebastian
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Hauswaldt, J. Susanne;Polok, Andy;Steinfartz, Sebastian

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我们描述了在现存最古老的火蜥蜴谱系中分离出的10个微卫星基因座,这是一种意大利亚平宁半岛特有的陆生火蜥蜴属。对北方物种(Salamandrina Perspicillata)和南方物种(Salamandrina Terdigitata)每个物种的24个个体进行了引物测试。所有座位均为多态,等位基因数在3~11个之间,杂合度在0.25~0.83之间。毛竹中有8个座位表现出多态,等位基因数在2~9个之间,杂合度在0.04~0.92之间。
We describe ten microsatellite loci isolated for the oldest lineage of extant salamandrids, the spectacled salamanders (Salamandrina), a genus of terrestrial salamanders endemic to the Apennine peninsula (Italy). Primers were tested in 24 individuals per species, the northern species (Salamandrina perspicillata) and the southern species (Salamandrina terdigitata). All loci were polymorphic in S. perspicillata with number of alleles ranging from three to eleven and observed heterozygosity ranging from 0.25 to 0.83. In S. terdigitata eight loci were polymorphic, the number of alleles ranged from two to nine, and observed heterozygosity from 0.04 to 0.92.