The SSV1 viral integrase is not essential
The SSV1 viral integrase is not essential
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DOI：
10.1016/j.virol.2006.11.003
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发表时间：
2007-04-25
期刊：
影响因子：
3.7
通讯作者：
Stedman, Kenneth M.
中科院分区：
文献类型：
--
作者：
Clore, Adam J.;Stedman, Kenneth M.
病毒整合是病毒在所有生命领域中广泛保守的特征;然而，在许多情况下，其必要性并没有得到很好的理解。使用酪氨酸重组酶的整合是最广泛和最好表征的整合机制之一。我们完全删除了酪氨酸重组酶整合酶的极端嗜热和嗜酸性古菌病毒SSV 1使用一种新的LIPCR技术，发现该病毒仍然复制和传播在其宿主硫磺硫化叶菌没有整合。突变体病毒保持持续感染，但整合酶缺乏病毒的竞争力比野生型病毒时，共培养。基于这些结果，我们讨论了集成的必要性和这种类型的复制策略的可能优势。(c)2006年爱思唯尔公司All rights reserved.
Viral integration is a widely conserved characteristic in viruses in all domains of life; however, its necessity is not well understood in many cases. Integration using tyrosine recombinases is one of the most widespread and best characterized mechanisms of integration. We completely removed the tyrosine recombinase integrase from the hyperthermophilic and acidophilic archaeal virus SSV1 using a novel LIPCR technique and found that the virus still replicated and spread in its host Sulfolobus solfataricus without integration. The mutant virus maintained a persistent infection but the integrase-lacking virus was less competitive than the wild-type virus when co-cultured. Based on these results, we discuss the necessity of integration and the possible advantages of this type of replication strategy. (c) 2006 Elsevier Inc. All rights reserved.