Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage
Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage
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DOI：
10.1093/femsle/fnw211
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发表时间：
2016-10-01
影响因子：
2.1
通讯作者：
Pantucek, Roman
中科院分区：
文献类型：
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作者：
Maslanova, Ivana;Stribna, Sabina;Pantucek, Roman
噬菌体介导的基因转导被认为是葡萄球菌水平基因转移的主要驱动力之一，对葡萄球菌的适应和成功进化至关重要。为了使转导有效，通常认为受体菌株应该对转导噬菌体敏感。在这项研究中，我们证明了质粒DNA被有效地转导到受体金黄色葡萄球菌菌株，尽管它们对转导噬菌体的裂解作用不敏感，只要这些噬菌体有效地吸附到细菌细胞。通过噬菌体phi 29、phi 52 A和phi 80 α以及原噬菌体phi 53和从供体溶原性菌株诱导的天然存在的原噬菌体，将四环素和青霉素酶质粒转导至不敏感的实验室和临床菌株。在噬菌体敏感和噬菌体不敏感的受体菌株中实现了相当的转导频率。我们已经证明，DNA的限制和溶原性免疫等机制，负责细胞对葡萄球菌的不敏感性，可能不是一个障碍，转移，维持和有效传播的质粒到更广泛的潜在受体的葡萄球菌种群。
The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer in staphylococci, which is crucial to their adaptation and successful evolution. For a transduction to be effective, it is generally accepted that the recipient strain should be susceptible to the transducing phage. In this study, we demonstrate that the plasmid DNAs are effectively transduced into the recipient Staphylococcus aureus strains in spite of their insensitivity to the lytic action of the transducing phage, provided that these phages adsorb effectively to the bacterial cells. The tetracycline and penicillinase plasmids were transduced to insensitive laboratory and clinical strains by bacteriophages phi 29, phi 52A and phi 80 alpha as well as by prophage phi 53 and naturally occurring prophages induced from donor lysogenic strains. Comparable frequencies of transduction were achieved in both phage-sensitive and phage-insensitive recipient strains. We have demonstrated that such mechanisms as the restriction of DNA and lysogenic immunity which are responsible for insensitivity of cells to phages may not be a barrier to the transfer, maintenance and effective spread of plasmids to a wider range of potential recipients in the staphylococcal population.