On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves
On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves
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DOI：
10.1073/pnas.1209288109
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发表时间：
2012-07-10
影响因子：
11.1
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Ding, Xiaoyun;Lin, Sz-Chin Steven;Huang, Tony Jun
能够巧妙地操纵单个粒子、细胞和生物体的技术在生物、化学、工程和物理中的许多应用中都是无价的。在这里，我们展示了基于立式表面声波的“声波镊子”，它可以在单层微流控芯片中捕获和操纵单个微粒、细胞和整个生物体(即秀丽线虫)。我们的声镊子利用线性调频叉指换能器的宽共振频带来实现对静止表面声波场的实时控制，从而能够灵活地操纵大多数已知的微粒。我们的声学设备所需的功率密度明显低于光学设备(比光学镊子少1000万倍，比光电子镊子少100倍)，这使得这项技术更具生物兼容性，更易于微型化。进行了细胞活性测试，以验证镊子与生物对象的兼容性。由于其在生物兼容性、小型化和通用性方面的优势，这里介绍的声波镊子将成为许多科学和工程学科的强大工具。
Techniques that can dexterously manipulate single particles, cells, and organisms are invaluable for many applications in biology, chemistry, engineering, and physics. Here, we demonstrate standing surface acoustic wave based "acoustic tweezers" that can trap and manipulate single microparticles, cells, and entire organisms (i.e., Caenorhabditis elegans) in a single-layer microfluidic chip. Our acoustic tweezers utilize the wide resonance band of chirped interdigital transducers to achieve real-time control of a standing surface acoustic wave field, which enables flexible manipulation of most known microparticles. The power density required by our acoustic device is significantly lower than its optical counterparts (10,000,000 times less than optical tweezers and 100 times less than optoelectronic tweezers), which renders the technique more biocompatible and amenable to miniaturization. Cell-viability tests were conducted to verify the tweezers' compatibility with biological objects. With its advantages in biocompatibility, miniaturization, and versatility, the acoustic tweezers presented here will become a powerful tool for many disciplines of science and engineering.