A spontaneous wheat-Aegilops longissima translocation carrying Pm66 confers resistance to powdery mildew
A spontaneous wheat-Aegilops longissima translocation carrying Pm66 confers resistance to powdery mildew
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携带 Pm66 的自发小麦-长山羊草易位赋予对白粉病的抗性
DOI：
10.1007/s00122-020-03538-8
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发表时间：
2020-04-01
影响因子：
5.4
通讯作者：
Liu, Wenxuan
中科院分区：
文献类型：
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作者：
Li, Huanhuan;Dong, Zhenjie;Liu, Wenxuan
发现了一条携带Pm 66的自发罗伯逊T4 S（l）S中心点4 BL易位染色体，该染色体具有白粉病抗性，并通过RNA-seq、分子标记和原位杂交分析证实。小麦白粉病是由小麦白粉病菌Blumeria graminis f.小麦赤霉病（Bgt）是一种世界性的小麦病害。小麦近缘野生种抗白粉病基因的发现和利用在小麦改良中发挥了重要作用。长山羊草是小麦的S基因组二倍体野生近缘种之一，是小麦抗病虫的重要资源。Ae.的4S（l）染色体。longissima赋予对白粉病的中等抗性。在这项研究中，我们进行了RNA-seq的推定中国春天（CS）-Ae。longissima 4S（1）（4 B）二体代换系（TA 3465）中的Bgt抗性基因，以开发4S（1）特异性标记，从而帮助通过诱导的同源重组从4S（1）转移Bgt抗性基因。CS和TA 3465之间的基因的成对比较表明，CS中染色体4 BS上的许多基因在TA 3465中不表达。4 B和4S（1）特异性分子标记分析表明，4 BS和4S（1）L在TA 3465中均缺失，而4 BL和4S（1）S存在。通过基因组和荧光原位杂交的进一步表征证实了TA 3465携带自发的罗伯逊T4 S（I）S中心点4 BL易位。白粉病测定结果表明，TA 3465对16个Bgt菌株中的10个菌株表现抗性，而CS对所有菌株均表现感病。将TA 3465中的白粉病抗性基因进一步定位到4S（1）的短臂上，并命名为Pm 66。
Key message A spontaneous Robertsonian T4S(l)S center dot 4BL translocation chromosome carrying Pm66 for powdery mildew resistance was discovered and confirmed by RNA-seq, molecular marker, and in situ hybridization analyses. Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a severe disease of bread wheat worldwide. Discovery and utilization of resistance genes to powdery mildew from wild relatives of wheat have played important roles in wheat improvement. Aegilops longissima, one of the S-genome diploid wild relatives of wheat, is a valuable source of disease and pest resistance for wheat. Chromosome 4S(l) from Ae. longissima confers moderate resistance to powdery mildew. In this study, we conducted RNA-seq on a putative Chinese Spring (CS)-Ae. longissima 4S(l)(4B) disomic substitution line (TA3465) to develop 4S(l)-specific markers to assist the transfer of a Bgt resistance gene from 4S(l) by induced homoeologous recombination. A pairwise comparison of genes between CS and TA3465 demonstrated that a number of genes on chromosome 4BS in CS were not expressed in TA3465. Analysis of 4B- and 4S(l)-specific molecular markers showed that 4BS and 4S(l)L were both missing in TA3465, whereas 4BL and 4S(l)S were present. Further characterization by genomic and fluorescent in situ hybridization confirmed that TA3465 carried a spontaneous Robertsonian T4S(l)S center dot 4BL translocation. Powdery mildew tests showed that TA3465 was resistant to 10 of 16 Bgt isolates collected from different regions of China, whereas CS was susceptible to all those Bgt isolates. The powdery mildew resistance gene(s) in TA3465 was further mapped to the short arm of 4S(l) and designated as Pm66.