The dynamics of repeated elements: applications to the epidemiology of tuberculosis.
The dynamics of repeated elements: applications to the epidemiology of tuberculosis.
复制标题
重复元素的动力学：在结核病流行病学中的应用。
DOI：
10.1073/pnas.97.7.3532
复制
发表时间：
2000
影响因子：
11.1
通讯作者：
Feldman,MW
中科院分区：
文献类型：
--
作者：
Tanaka,MM;Small,PM;Salamon,H;Feldman,MW
We propose a stepwise mutation model to describe the dynamics of DNA fingerprint variation inMycobacterium tuberculosis. The genome ofM. tuberculosiscarries insertion sequences (IS6110) that are relatively stable over time periods of months but have an observable transposition rate over longer time scales. Variability in copy number and genomic location of (IS6110) can be harnessed to generate a DNA fingerprint for each strain, by digesting the genome with a restriction enzyme and using a portion of the element as a probe for Southern blots. The number of bands found for a given genome approximates the number of copies of IS6110it carries. A large data set of such fingerprints from tuberculosis (TB) cases in San Francisco provides an observed distribution of IS6110copy number. Implementation of the model through deterministic and stochastic simulation indicates some general features of IS/TB dynamics. By comparing observations with outcomes of the model, we conclude that the IS/TB system is very heterogeneous and far from equilibrium. We find that the transposition parameters have a much stronger effect than the epidemic parameters on copy number distribution.
